Identification of Candida species isolated from cancer patients by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism in Apeksha Hospital, Maharagama, Sri Lanka

被引：0

作者：

Ganguli, Sammarappulige Chathma ^{[1
]}

Wijendra, Somalatha ^{[2
]}

Dasanayaka, Nilanthie ^{[1
]}

Ratnasingham, Ramesh ^{[2
]}

Kaushalya, Gaya ^{[2
]}

Gunasekera, Samanmalee Priyantha ^{[3
]}

机构：

[1] Univ Sri Jayewardenepura, Dept Bot, Nugegoda, Sri Lanka

[2] Med Res Inst, Colombo, Sri Lanka

[3] Apeksha Hosp, Microbiol Lab, Mahargama, Sri Lanka

来源：

CEYLON MEDICAL JOURNAL | 2023年 / 68卷 / 03期

关键词：

Candida; PCR-RFLP; cancer patients; PCR-RFLP;

D O I：

10.4038/cmj.v68i3.9928

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Candida infections pose a significant challenge in managing cancer patients. A rapid and effective method for Candida species identification is crucial to address this issue. This study at Apeksha Hospital, Maharagama, aimed to identify Candida species using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism. ITS1-5.8S-ITS2 rDNA regions of 52 clinical Candida isolates and standard strains were amplified using ITS1 and ITS4 primers and digested with MspI enzyme. Candida tropicalis (38%), Candida parapsilosis (31%), Candida albicans (13%), Candida glabrata (8%), Candida krusei (4%), Candida haemulonii (4%), and Candida intermedia (2%) were identified. PCRRFLP proves to be a rapid, and reliable method for identifying medically important Candida species in cancer patients.

引用

页码：152 / 155

页数：4

共 10 条

[1] BROWN AE, 1990, SEMIN ONCOL, V17, P2
[2] Identification of Candida Species Isolated from Vulvovaginal Candidiasis Patients by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) in Yasuj Southwestern Iran
Gharaghani, Maral
Ahmadi, Bahram
Sisakht, Marzie Taheripour
Ilami, Owrange
Aramesh, Shahintaj
Mouhamadi, Fariba
Barati, Zohreh
Roozmeh, Shohreh
Mohammadi, Haniyeh
Nouripour-Sisakht, Sadegh
[J]. JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2018, 11 (08)
[3] Jafari Zahra, 2017, Curr Med Mycol, V3, P10, DOI 10.29252/cmm.3.3.10
[4] Candidemia in the cancer patient: diagnosis, treatment, and future directions
McCarthy, Matthew W.
Walsh, Thomas J.
[J]. EXPERT REVIEW OF ANTI-INFECTIVE THERAPY, 2018, 16 (11) : 849 - 854
[5] Mirhendi Hossein, 2006, Nihon Ishinkin Gakkai Zasshi, V47, P225, DOI 10.3314/jjmm.47.225
[6] Molecular identification and distribution profile of Candida species isolated from Iranian patients
Mohammadi, Rasoul
Mirhendi, Hossein
Rezaei-Matehkolaei, Ali
Ghahri, Mohammad
Shidfar, Mohammad Reza
Jalalizand, Nilufar
Makimura, Koichi
[J]. MEDICAL MYCOLOGY, 2013, 51 (06) : 657 - 663
[7] Identification of Candida Species Isolated From Oral Colonization in Iranian HIV-Positive Patients, by PCR-RFLP Method.
Mousavi, Seyyed Amin Ayatollahi
Salari, Samira
Rezaie, Sasan
Nejad, Naser Shahabi
Hadizadeh, Sanaz
Kamyabi, Hossein
Aghasi, Hossein
[J]. JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2012, 5 (01) : 336 - 340
[8] Identification of Candida species using PCR-RFLP in cancer patients in Iran
Shokohi, T.
Soteh, M. B. Hashemi
Pouri, Z. Saltanat
Hedayati, M. T.
Mayahi, S.
[J]. INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 28 (02) : 147 - 151
[9] Silva Danielly BS, 2017, International Journal of Genetic Science
[10] Wasak-Szulkowska Ewa, 2005, Pol Merkur Lekarski, V19, P5

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