Sequential Activation of DNA Sensor Enables Correlated Imaging of Dual-Enzyme Activities in Living Cells

被引:0
|
作者
Wang, Xian [1 ]
Yi, Deyu [1 ]
Li, Mengyuan [1 ]
Li, Zhengping [1 ]
机构
[1] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing Key Lab Bioengn & Sensing Technol, Beijing 100083, Peoples R China
基金
中国国家自然科学基金;
关键词
FLAP ENDONUCLEASE-1; CISPLATIN; TARGET; GENE;
D O I
10.1021/acs.analchem.4c05454
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The DNA repair system relies on the coordinated action of multiple enzymes to maintain genomic stability, with apurinic/apyrimidinic endonuclease 1 (APE1) and flap endonuclease 1 (FEN1) playing pivotal roles in the long-patch base excision repair (LP-BER) pathway. Elevated levels of APE1 and FEN1 have been associated with tumor progression and resistance to therapy, making them key biomarkers for cancer diagnosis and treatment monitoring. Here, we present a sequentially activated AND-logic DNA sensor (D-AF) for the correlated imaging of APE1 and FEN1 in living cells. The sensor operates through a sequential process: APE1 first recognizes and cleaves an apurinic site, initiating structural changes that enable FEN1 to cleave a 5 ' flap, resulting in restored fluorescence. We demonstrate the use of the D-AF-based nanosensor for in situ imaging of APE1 and FEN1 activities in cancer cells and for monitoring of enzyme dynamics during chemotherapy. This platform offers a valuable tool for investigating DNA repair mechanisms and their roles in cancer diagnosis and treatment.
引用
收藏
页码:4373 / 4378
页数:6
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