共 38 条
Sequential Activation of DNA Sensor Enables Correlated Imaging of Dual-Enzyme Activities in Living Cells
被引:0
|作者:
Wang, Xian
[1
]
Yi, Deyu
[1
]
Li, Mengyuan
[1
]
Li, Zhengping
[1
]
机构:
[1] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing Key Lab Bioengn & Sensing Technol, Beijing 100083, Peoples R China
基金:
中国国家自然科学基金;
关键词:
FLAP ENDONUCLEASE-1;
CISPLATIN;
TARGET;
GENE;
D O I:
10.1021/acs.analchem.4c05454
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
The DNA repair system relies on the coordinated action of multiple enzymes to maintain genomic stability, with apurinic/apyrimidinic endonuclease 1 (APE1) and flap endonuclease 1 (FEN1) playing pivotal roles in the long-patch base excision repair (LP-BER) pathway. Elevated levels of APE1 and FEN1 have been associated with tumor progression and resistance to therapy, making them key biomarkers for cancer diagnosis and treatment monitoring. Here, we present a sequentially activated AND-logic DNA sensor (D-AF) for the correlated imaging of APE1 and FEN1 in living cells. The sensor operates through a sequential process: APE1 first recognizes and cleaves an apurinic site, initiating structural changes that enable FEN1 to cleave a 5 ' flap, resulting in restored fluorescence. We demonstrate the use of the D-AF-based nanosensor for in situ imaging of APE1 and FEN1 activities in cancer cells and for monitoring of enzyme dynamics during chemotherapy. This platform offers a valuable tool for investigating DNA repair mechanisms and their roles in cancer diagnosis and treatment.
引用
收藏
页码:4373 / 4378
页数:6
相关论文