Chiral pesticide permethrin promotes the antibiotic resistance genes dissemination by transformation: Different chiral isomers engage in distinct regulatory pathways

被引：0
作者：
Li, Chenxi [1 ]
Wang, Linjie [1 ]
Dai, Qi [1 ]
Chong, Yang [2 ]
Utsunomiya, Satoshi [3 ]
Wang, Honggui [1 ]
Zhang, Ya [1 ,4 ]
Han, Jie [5 ]
机构：
[1] Yangzhou Univ, Sch Environm Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China
[2] Yangzhou Univ, Affiliated Hosp, Dept Tradit Chinese Med, Yangzhou 225000, Jiangsu, Peoples R China
[3] Kyushu Univ, Dept Chem Engn, 744 Motooka,Nishi Ku, Fukuoka 8190395, Japan
[4] Yangzhou Univ, Inst Technol Carbon Neutralizat, Sch Environm Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China
[5] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225009, Peoples R China
来源：
JOURNAL OF HAZARDOUS MATERIALS | 2025年 / 488卷
基金：
中国国家自然科学基金;
关键词：
Chiral pesticides; Permethrin; Antibiotic resistance genes; Chiral isomers; Natural transformation; OXIDATIVE STRESS; BACTERIAL; DEGRADATION; COMPETENCE; EXPRESSION; TOXICITY; SYSTEM; SOIL;
D O I：
10.1016/j.jhazmat.2025.137416
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The global dissemination of antibiotic resistance genes (ARGs) poses an increasingly urgent threat to both environmental and human health. The extensive use of chiral permethrin (PM), the most popular synthetic type I pyrethroid insecticide worldwide, has led to its increased detection in aquatic environments. However, our understanding of PM's role in spreading ARGs is still limited. Here, we systematically assessed the effects of two chiral isomers of 1R-cis-PM (CPM) and 1R-trans-PM (TPM) on the dissemination of ARGs in the aquatic environments by using a natural transformation (NT) model comprising plasmid pWH1274 and Acinetobacter baylyi ADP1. It was found that reactive oxygen species (ROS) was the main factor facilitating the NT of ARGs mediated by CPM and TPM, although their respective production mechanisms exhibited distinct pathways: CPM generates ROS primarily through the primary electron transport chain (ETC), whereas TPM does so via a secondary ETC. Furthermore, CPM enhanced NT by improving the bacterial competent state, while TPM promotes it by enhancing recombination. It was confirmed that both CPM and TPM have the potential to accelerate the spread of ARGs through distinct mechanisms. These findings will help us understand that different chiral isomers may pose risks through distinct mechanisms.
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页数：11
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