A PCR-Based Approach for Early Diagnosis of Head and Neck Aspergillosis: A Pilot Study

被引:0
作者
Gomes, Thais Ellen Chaves [1 ]
Bastos, Victor Coutinho [2 ]
Boniek, Douglas [3 ]
Romanach, Mario [4 ]
Rocha, Fernanda Faria [1 ]
Chaves, Roberta Rayra Martins [5 ]
Gomez, Ricardo Santiago [1 ,5 ]
机构
[1] Univ Fed Minas Gerais, Sch Dent, Dept Oral Surg & Pathol, BR-31270901 Belo Horizonte, Brazil
[2] Univ Fed Minas Gerais, Biol Sci Inst, Dept Pathol, BR-31270901 Belo Horizonte, Brazil
[3] Univ Fed Minas Gerais, Biol Sci Inst, Dept Microbiol, BR-31270901 Belo Horizonte, Brazil
[4] Univ Fed Rio Janeiro, Sch Dent, Dept Oral Pathol, BR-21941913 Rio De Janeiro, Brazil
[5] Fac Ciencias Med Minas Gerais, Med Sch, BR-30130110 Belo Horizonte, Brazil
关键词
aspergillosis; Aspergillus; PCR; diagnosis; INFECTIOUS-DISEASES;
D O I
10.3390/genes15111428
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Background: Aspergillosis is a fungal disease caused by the inhalation of fungal spores of the genus Aspergillus spp. This fungus mainly affects the lungs but can spread and infect the maxillofacial region through the bloodstream or inoculation of the fungus after extraction or endodontic treatment, especially in the upper posterior teeth. The disease has nonspecific clinical manifestations that hinder its early diagnosis. Although the Polymerase Chain Reaction (PCR) technique holds promise as a diagnostic tool for aspergillosis, anatomopathological analysis services do not routinely adopt this method. Objectives: Therefore, the present study aimed to evaluate the applicability of PCR and standardise the techniques of preparation of biological samples for the detection of the three species: Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus. Methods: Six samples of formalin-fixed, paraffin-embedded tissue (FFPE) with a histopathological diagnosis suggestive of aspergillosis were investigated using PCR. As a positive control for the PCR reaction, morphologically and genetically characterized cultures were used, with their sequences deposited at NCBI under accession codes MW837777 (A. fumigatus) and MW837779 (A. niger). The A. flavus culture used is reference RC 2053. Results: Four of the six samples evaluated were positive for Aspergillus spp., of which one was co-infected with A. fumigatus and A. flavus species, while two others were positive only for A. flavus, and one sample was positive only for A. fumigatus. Conclusions: These findings suggest that PCR can be used as an auxiliary method for diagnosing aspergillosis. However, this was a pilot study, and expansion of the sample size and the evaluation of PCR in comparison with other diagnostic tests for aspergillosis are essential to determine the accuracy of the method.
引用
收藏
页数:9
相关论文
共 24 条
[1]   Dermatologic Urgencies and Emergencies What Every Pathologist Should Know [J].
Abate, Mallory S. ;
Battle, Laura R. ;
Emerson, Ashley N. ;
Gardner, Jerad M. ;
Shalin, Sara C. .
ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2019, 143 (08) :919-942
[2]   Phenotypic and genotypic identification of human pathogenic aspergilli [J].
Balajee, S. Arunmozhi ;
Marr, Kieren A. .
FUTURE MICROBIOLOGY, 2006, 1 (04) :435-445
[3]  
Batista P.P., 2007, Caracterizacao de Linhagens do grupo Aspergillus flavus Baseada em Marcadores de DNA
[4]  
Bennett Joan W., 2010, ASP MOL BIOL GEN, P1
[5]  
Bongomin F, 2017, J FUNGI, V3, DOI [10.3390/jof3040057, 10.3390/jof3020026]
[6]   Aspergillosis Epidemiology, Diagnosis, and Treatment [J].
Cadena, Jose ;
Thompson, George R., III ;
Patterson, Thomas F. .
INFECTIOUS DISEASE CLINICS OF NORTH AMERICA, 2021, 35 (02) :415-434
[7]   Tendency in Pulmonary Aspergillosis Investigation during the COVID-19 Era: What Is Changing? [J].
Caggiano, Giuseppina ;
Apollonio, Francesca ;
Consiglio, Mila ;
Gasparre, Valentina ;
Trerotoli, Paolo ;
Diella, Giusy ;
Lopuzzo, Marco ;
Triggiano, Francesco ;
Stolfa, Stefania ;
Mosca, Adriana ;
Montagna, Maria Teresa .
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2022, 19 (12)
[8]   Multiplex PCR Based Strategy for Detection of Fungal Pathogen DNA in Patients with Suspected Invasive Fungal Infections [J].
Carvalho-Pereira, Joana ;
Fernandes, Filipa ;
Araujo, Ricardo ;
Springer, Jan ;
Loeffler, Juergen ;
Buitrago, Maria Jose ;
Pais, Celia ;
Sampaio, Paula .
JOURNAL OF FUNGI, 2020, 6 (04) :1-16
[9]   Blood Aspergillus PCR: The Good, the Bad, and the Ugly [J].
Egger, Matthias ;
Jenks, Jeffrey D. ;
Hoenigl, Martin ;
Prattes, Juergen .
JOURNAL OF FUNGI, 2020, 6 (01)
[10]   Aspergillus in endodontic infection near the maxillary sinus [J].
Gomes, Cinthya Cristina ;
Costa Pinto, Larissa Christina ;
Victor, Fernanda Loretti ;
Borges da Silva, Erlange Andrade ;
Ribeiro, Apoena de Aguiar ;
de Moura Sarquis, Maria Ines ;
Gomes Camoes, Isabel Coelho .
BRAZILIAN JOURNAL OF OTORHINOLARYNGOLOGY, 2015, 81 (05) :527-532