Zebrafish as a model for the study of wound healing in hyperglycemia

被引:0
|
作者
Nurkhasanah, Lia [1 ]
Hayati, Farida [1 ]
Istikharah, Rochmy [1 ]
机构
[1] Univ Islam Indonesia, Dept Pharm, Yogyakarta, Indonesia
来源
PHARMACY EDUCATION | 2024年 / 24卷 / 03期
关键词
Animal model; Gene; Hyperglycemia; Regeneration; Wound healing; Zebrafish;
D O I
10.46542/pe.2024.243.111115
中图分类号
G40 [教育学];
学科分类号
040101 ; 120403 ;
摘要
Background: For preclinical studies of diabetic wound therapy, zebrafish have many orthologous signalling pathways that play essential roles in wound healing and regeneration. Objectives: This study compares the expression of four essential wound- healing genes and caudal fin regeneration in hyperglycemic zebrafish ( Danio rerio) ) to normal zebrafish. Methods: Hyperglycemia was induced by using the intraperitoneal injection method with 350 mg/BW streptozotocin on days one, three, and five. The regeneration of the zebrafish's caudal fin was observed on day 5 after amputation using a stereomicroscope, followed by sampling of the blastema to analyse gene expression. Caudal fin regeneration was analysed using Zeiss Zen 3.3 blue documentation; blood glucose levels were measured using a glucometer; and relative gene expression analysis of sonic hedgehog (shh), insulin-like growth factor 2a (igf2a), bone morphogenetic protein 2b (bmp2b), collagen 1a2 (col1a2) was performed by qRT-PCR method. Results: The glucose level in the hyperglycemia group was 203.2 mg/dL, and that in the normal group was 59.5 mg/dL. The authors found that i gf2a, shh, bmp2b, and col1a2 expression were all downregulated in the hyperglycemia group and decreased in caudal fin regeneration. Conclusion: This novel adult zebrafish model of hyperglycemia significantly impairs gene expression and caudal fin regeneration.
引用
收藏
页码:111 / 115
页数:5
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