Molecular characterization and subtype analysis of Blastocystis sp. in captive wildlife in Henan, China

被引：0
作者：
Cui, Zhaohui [1 ,4 ,5 ]
Huang, Xiyao [2 ]
Zhang, Sulan [1 ]
Li, Kaifang [3 ]
Zhang, Aili [1 ]
Li, Qichen [1 ]
Zhang, Yutong [1 ]
Li, Junqiang [4 ]
Qi, Meng [5 ]
机构：
[1] Xuchang Univ, Food & Pharm Coll, Key Lab Biomarker Based Rapid Detect Technol Food, Xuchang 461000, Peoples R China
[2] Xuchang Univ, Coll Chem & Mat Engn, Xuchang 461000, Peoples R China
[3] Luohe Anim Dis Prevent & Control Ctr, Luohe 462000, Peoples R China
[4] Henan Agr Univ, Coll Vet Med, 218 Ping Ave, Zhengzhou 450046, Peoples R China
[5] Tarim Univ, Coll Anim Sci & Technol, Alar 843300, Peoples R China
来源：
PARASITE | 2025年 / 32卷
关键词：
Blastocystis sp; Captive wildlife; Subtypes; Zoonotic potential; ANIMALS; BIRDS;
D O I：
10.1051/parasite/2025006
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Blastocystis sp. is a zoonotic intestinal protozoan that is ubiquitous globally, residing in the gastrointestinal tracts of both humans and various animals. In the present study, a PCR-sequencing tool based on the SSU rRNA gene was employed to investigate the prevalence and subtypes of Blastocystis spp. in 204 fresh fecal samples collected from 20 captive wildlife species from a bird park in Henan Province, Central China. Overall, Blastocystis was present in 13.73% (28 out of 204) of the samples and 25% (5 out of 20) of the species. A total of four zoonotic subtypes of Blastocystis sp. were found: ST1, ST3, ST5, and ST27, with the latter being the most prevalent, accounting for 35.71% (10 out of 28) of the 5 species positive for Blastocystis sp. To the best of our knowledge, this is the first report of Blastocystis ST27 in birds in China, namely bar-headed goose (Anser indicus) and peafowl (Pavo muticus). The data suggest that captive wildlife, particularly those in bird parks, may frequently be infected with this zoonotic pathogen. Consequently, these animals may serve as potential reservoirs for zoonotic infections in humans. Blastocystis sp. est un protozoaire intestinal zoonotique omnipr & eacute;sent dans le monde entier, r & eacute;sidant dans le tractus gastro-intestinal des humains et de divers animaux. Dans la pr & eacute;sente & eacute;tude, un outil de s & eacute;quen & ccedil;age par PCR bas & eacute; sur le g & egrave;ne SSU rRNA a & eacute;t & eacute; utilis & eacute; pour & eacute;tudier la pr & eacute;valence et les sous-types de Blastocystis spp. dans 204 & eacute;chantillons de mati & egrave;res f & eacute;cales fra & icirc;ches pr & eacute;lev & eacute;s sur 20 esp & egrave;ces sauvages en captivit & eacute; dans un parc ornithologique de la province du Henan, en Chine centrale. Dans l'ensemble, Blastocystis & eacute;tait pr & eacute;sent dans 13,73 % (28 sur 204) des & eacute;chantillons et dans 25 % (5 sur 20) des esp & egrave;ces. Au total, quatre sous-types zoonotiques de Blastocystis sp. ont & eacute;t & eacute; trouv & eacute;s : ST1, ST3, ST5 et ST27, ce dernier & eacute;tant le plus r & eacute;pandu, repr & eacute;sentant 35,71% (10 sur 28) des 5 esp & egrave;ces positives pour Blastocystis sp. & Agrave; notre connaissance, il s'agit du premier signalement de Blastocystis ST27 chez des oiseaux en Chine, & agrave; savoir l'oie & agrave; t & ecirc;te barr & eacute;e (Anser indicus) et le paon (Pavo muticus). Les donn & eacute;es sugg & egrave;rent que la faune en captivit & eacute;, en particulier celle des parcs ornithologiques, peut & ecirc;tre fr & eacute;quemment infect & eacute;e par ce pathog & egrave;ne zoonotique. Par cons & eacute;quent, ces animaux peuvent servir de r & eacute;servoirs potentiels d'infections zoonotiques pour l'homme.
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