Large-scale phenotypic and genomic analysis of Listeria monocytogenes reveals diversity in the sensitivity to quaternary ammonium compounds but not to peracetic acid

被引：0

作者：

Ivanova, Mirena ^{[1
]}

Kragh, Martin Laage

Szarvas, Judit ^{[1
]}

Tosun, Elif Seyda ^{[1
]}

Holmud, Natacha Friis ^{[1
]}

Gmeiner, Alexander ^{[1
]}

Amar, Corinne ^{[3
]}

Guldimann, Claudia ^{[4
]}

Huynh, TuAnh N. ^{[5
]}

Karpiskova, Renata ^{[6
]}

Rota, Carmen ^{[7
]}

Gomez, Diego ^{[7
]}

Aboagye, Eurydice ^{[8
]}

Etter, Andrea ^{[8
]}

Centorame, Patrizia ^{[9
]}

Torresi, Marina ^{[9
]}

De Angelis, Maria Elisabetta ^{[9
]}

Pomilio, Francesco ^{[9
]}

Okholm, Anders Hauge ^{[10
]}

Xiao, Yinghua ^{[10
]}

Kleta, Sylvia ^{[11
]}

Lueth, Stefanie ^{[11
]}

Pietzka, Ariane ^{[12
]}

Kovacevic, Jovana ^{[13
]}

Pagotto, Franco ^{[14
]}

Rychli, Kathrin ^{[15
]}

Zdovc, Irena ^{[16
]}

Papic, Bojan ^{[16
]}

Heir, Even ^{[17
]}

Langsrud, Solveig ^{[17
]}

Moretro, Trond ^{[17
]}

Brown, Phillip ^{[18
]}

Kathariou, Sophia ^{[18
]}

Stephan, Roger ^{[19
]}

Tasara, Taurai ^{[19
]}

Dalgaard, Paw ^{[2
]}

Njage, Patrick Murigu Kamau ^{[1
]}

Fagerlund, Annette ^{[18
]}

Aarestrup, Frank ^{[1
]}

Hansen, Lisbeth Truelstrup ^{[2
]}

Leekitcharoenphon, Pimlapas ^{[1
]}

机构：

[1] Tech Univ Denmark, Natl Food Inst, Res Grp Genom Epidemiol, Lyngby, Denmark

[2] Tech Univ Denmark, Natl Food Inst, Res Grp Food Microbiol & Hyg, Lyngby, Denmark

[3] Publ Hlth England, Natl Infect Serv, London, England

[4] Ludwig Maximilians Univ Munchen, Chair Food Safety & Analyt, Munich, Germany

[5] Univ Wisconsin, Madison, WI USA

[6] Masaryk Univ, Dept Publ Hlth, Med Fac, Brno, Czech Republic

[7] Univ Zaragoza, Zaragoza, Spain

[8] Univ Vermont, Burlington, VT USA

[9] Ist Zooprofilatt Sperimentale Abruzzo & Molise G C, Teramo, Italy

[10] Arla Foods Amba, Arla Innovat Ctr, Aarhus, Denmark

[11] German Fed Inst Risk Assessment BfR, Natl Reference Lab Listeria Monocytogenes NRL Lm, Berlin, Germany

[12] Austrian Agcy Hlth & Food Safety AGES, Inst Med Microbiol & Hyg, Natl Reference Lab Listeria Monocytogenes, Graz, Austria

[13] Oregon State Univ, Food Innovat Ctr, Portland, OR USA

[14] Bur Microbial Hazards, Food Directorate, Listeriosis Reference Serv, Ottawa, ON, Canada

[15] Univ Vet Med, Inst Food Safety Food Technol & Vet Publ Hlth, Unit Food Microbiol, Vienna, Austria

[16] Univ Ljubljana, Inst Microbiol & Parasitol, Vet Fac, Ljubljana, Slovenia

[17] Norwegian Inst Food & Aquaculture Res, Fisheries & Aquaculture Res, As, Norway

[18] North Carolina State Univ, Raleigh, NC USA

[19] Univ Zurich, Inst Food Safety & Hyg, Vetsuisse Fac, Zurich, Switzerland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2025年

关键词：

Listeria monocytogenes; food industry; disinfectants; quaternary ammonium compounds; peracetic acid; R PACKAGE; DISINFECTANTS; RESISTANCE; TOOL; ANTISEPTICS; ADAPTATION; TOLERANCE;

D O I：

10.1128/aem.01829-24

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Listeria monocytogenes presents a significant concern for the food industry due to its ability to persist in the food processing environment. One of the factors contributing to its persistence is decreased sensitivity to disinfectants. Our objective was to assess the diversity of L. monocytogenes sensitivity to food industry disinfectants by testing the response of 1,671 L. monocytogenes isolates to quaternary ammonium compounds (QACs) and 414 isolates to peracetic acid (PAA) using broth microdilution and growth curve analysis assays, respectively, and to categorize the isolates into sensitive and tolerant. A high phenotype-genotype concordance (95%) regarding tolerance to QACs was obtained by screening the genomes for the presence of QAC tolerance-associated genes bcrABC, emrE, emrC, and qacH. Based on this high concordance, we assessed the QAC genes' dissemination among publicly available L. monocytogenes genomes (n = 39,196). Overall, QAC genes were found in 23% and 28% of the L. monocytogenes collection in this study and in the global data set, respectively. bcrABC and qacH were the most prevalent genes, with bcrABC being the most detected QAC gene in the USA, while qacH dominated in Europe. No significant differences (P > 0.05) in the PAA tolerance were detected among isolates belonging to different lineages, serogroups, clonal complexes, or isolation sources, highlighting limited variation in the L. monocytogenes sensitivity to this disinfectant. The present work represents the largest testing of L. monocytogenes sensitivity to important food industry disinfectants at the phenotypic and genomic level, revealing diversity in the tolerance to QACs while all isolates showed similar sensitivity to PAA.

引用

页数：24

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