Oral administration of Lactobacillus casei DG® after ileostomy closure in restorative proctocolectomy: a randomized placebo-controlled trial (microbiota and immune microenvironment in pouchitis-MEP1)

被引：2

作者：

Angriman, Imerio ^{[1
]}

Scarpa, Melania ^{[2
]}

Savarino, Edoardo ^{[3
]}

Patuzzi, Ilaria ^{[4
]}

Rigo, Alessandra ^{[3
]}

Kotsafti, Andromachi ^{[2
]}

Stepanyan, Astghik ^{[1
]}

Sciuto, Elisa ^{[1
]}

Celotto, Francesco ^{[1
]}

Negro, Silvia ^{[1
]}

Caruso, Antonino ^{[3
]}

Ruffolo, Cesare ^{[1
]}

Bardini, Romeo ^{[1
]}

Pucciarelli, Salvatore ^{[1
]}

Barberio, Brigida ^{[3
]}

Spolverato, Gaya ^{[1
]}

Zingone, Fabiana ^{[3
]}

D'Inca, Renata ^{[3
]}

Castagliuolo, Ignazio ^{[5
]}

Scarpa, Marco ^{[1
]}

机构：

[1] Univ Padua, Dept Surg Oncol & Gastroenterol Sci, Gen Surg Unit 3, DiSCOG, Padua, Italy

[2] IRCCS, Veneto Inst Oncol IOV, Immunol & Mol Oncol Diagnost, Padua, Italy

[3] Univ Padua, Dept Surg Oncol & Gastroenterol Sci DiSCOG, Gastroenterol Unit, Padua, Italy

[4] Eubiome Srl, Padua, Italy

[5] Univ Padua, Dept Mol Med DMM, Padua, Italy

来源：

GUT MICROBES | 2024年 / 16卷 / 01期

关键词：

Probiotics; microbiota; pouchitis; ileal pouch-anal anastomosis; inflammatory bowel disease; ULCERATIVE-COLITIS; ILEAL POUCH; MUCOSA;

D O I：

10.1080/19490976.2024.2423037

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Pouchitis is an idiopathic inflammatory disease that may occur in ileal pouches, and it can lead to ileal pouch failure. This was a single-center, randomized, double-blinded, placebo-controlled trial that assessed the effect of Lactobacillus casei (L. casei) DG (R), a probiotic strain, on the ileal pouch mucosa to determine the crosstalk between microbiota and mucosal immune system. Fifty-two patients undergoing restorative proctocolectomy were recruited and randomly assigned to receive a daily oral supplementation of L. casei DG (R) (n = 26) or placebo (n = 26) for 8 weeks from the ileostomy closure (T0) to a pouch endoscopy after 8 weeks (T1) and 1 year (T2). Ileal pouch mucosa samples were collected at T0, T1, and T2. At T1, the L. casei DG (R)-supplemented group showed a significant reduction of inflammatory cytokines levels compared to T0 baseline levels in the pouch mucosa, whereas in the placebo group cytokines levels resulted stable. In conclusion, probiotic manipulation of mucosal microbiota by L. casei DG (R)-supplementation after stoma closure in patients who underwent restorative proctocolectomy has a beneficial impact on the ileal pouch microenvironment. Registration number: NCT03136419 (http://www.clinicaltrials.gov).

引用

页数：14