Identification and characterization of an enhancer element regulating expression of Cdkn1c (p57 gene)

被引：0
|
作者：
Koga, Daisuke [1 ]
Nakayama, Shogo [1 ]
Higa, Tsunaki [1 ,2 ]
Nakayama, Keiichi I. [1 ,2 ]
机构：
[1] Kyushu Univ, Med Inst Bioregulat, Dept Mol & Cellular Biol, Fukuoka, Japan
[2] Inst Sci Tokyo, Anticanc Strategies Lab, Adv Res Initiat, Bunkyo Ku, Tokyo 1138510, Japan
来源：
GENES TO CELLS | 2024年 / 29卷 / 12期
基金：
日本学术振兴会;
关键词：
embryonic stem cell; enhancer; gene expression; p57; MICE LACKING; CELL-CYCLE; P57(KIP2); DIFFERENTIATION; INHIBITORS; CANCER; HYPERPLASIA; QUIESCENCE; P27(KIP1); ABLATION;
D O I：
10.1111/gtc.13173
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The mammalian p57 protein is a member of the CIP/KIP family of cyclin-dependent kinase inhibitors and plays an essential role in the development of multiple tissues during embryogenesis as well as in the maintenance of tissue stem cells in adults. Although several transcription factors have been implicated in regulating the p57 gene, cis-elements such as enhancers that regulate its expression have remained ill-defined. Here we identify a candidate enhancer for the mouse p57 gene (Cdkn1c) within an intron of the Kcnq1 locus by 4C-seq analysis in mouse embryonic stem cells (mESCs). Deletion of this putative enhancer region with the CRISPR-Cas9 system or its suppression by CRISPR interference resulted in a marked attenuation of Cdkn1c expression in differentiating mESCs. Our results thus suggest that this region may serve as an enhancer for the p57 gene during early mouse embryogenesis.
引用
收藏
页码：1264 / 1274
页数：11
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