Independent repeated mutations within the alphaviruses Ross River virus and Barmah Forest virus indicates convergent evolution and past positive selection in ancestral populations despite ongoing purifying selection

被引：0

作者：

Pyke, Alyssa T. ^{[1
]}

Wilson, Daniel J. ^{[2
,3
]}

Michie, Alice ^{[4
]}

Mackenzie, John S. ^{[5
]}

Imrie, Allison ^{[4
]}

Cameron, Jane ^{[1
]}

Doggett, Stephen L. ^{[6
]}

Haniotis, John ^{[6
]}

Herrero, Lara J. ^{[7
]}

Caly, Leon ^{[8
]}

Lynch, Stacey E. ^{[9
]}

Mee, Peter T. ^{[9
]}

Madzokere, Eugene T. ^{[7
]}

Ramirez, Ana L. ^{[10
,11
,12
]}

Paramitha, Devina ^{[13
]}

Hobson-Peters, Jody ^{[13
]}

Smith, David W. ^{[6
,14
]}

Weir, Richard ^{[15
]}

Sullivan, Mitchell ^{[16
]}

Druce, Julian ^{[8
]}

Melville, Lorna ^{[15
]}

Robson, Jennifer ^{[17
]}

Gibb, Robert ^{[18
]}

van den Hurk, Andrew F. ^{[1
]}

Duchene, Sebastian ^{[19
,20
]}

机构：

[1] Queensland Govt, Dept Hlth, Publ Hlth Virol Lab, Publ & Environm Hlth Reference Labs, POB 594, Coopers Plains, Qld, Australia

[2] Univ Oxford, Big Data Inst, Li Ka Shing Ctr Hlth Informat & Discovery, Oxford Populat Hlth, Old Rd Campus, Oxford OX3 7LF, England

[3] Univ Oxford, Dept Continuing Educ, 1 Wellington Sq, Oxford OX1 2JA, England

[4] Univ Western Australia, Sch Biomed Sci, 35 Stirling Highway, Perth, WA 6009, Australia

[5] Curtin Univ, Fac Hlth Sci, GPOBox U1987, Bentley, WA 6845, Australia

[6] Westmead Hosp, NSW Hlth Pathol, 166-174 Hawkesbury Rd Westmead, Sydney, NSW 2145, Australia

[7] Griffith Univ, Inst Glyc, Gold Coast Campus,1 Parklands Dr, Southport, Qld 4215, Australia

[8] Royal Melbourne Hosp, Peter Doherty Inst Infect & Immun, Victorian Infect Dis Reference Lab, 792 Elizabeth St, Melbourne, Vic 3000, Australia

[9] AgriBio, Ctr AgriBiosci, Agr Victoria Res, 5 Ring Rd, Bundoora, Vic 3083, Australia

[10] James Cook Univ, Coll Publ Hlth Med & Vet Sci, POB 6811, Cairns, Qld 4870, Australia

[11] James Cook Univ, Australian Inst Trop Hlth & Med, POB 6811, Cairns, Qld 4870, Australia

[12] Jackson Lab, 10 Discovery Dr Connecticut, Farmington, CT 06032 USA

[13] Univ Queensland, Sch Chem & Mol Biosci, Bdg 68 Cooper Rd, St Lucia, Qld 4072, Australia

[14] Univ Western Australia, Sch Med, 35 Stirling Highway, Perth, WA 6009, Australia

[15] Berrimah Vet Lab, Dept Primary Ind & Fisheries, POB 3000, Darwin, NT 0801, Australia

[16] Queensland Govt, Dept Hlth, Publ & Environm Hlth Reference Labs, POB 594 Archerfield, Coopers Plains, Qld 4108, Australia

[17] Sullivan Nicolaides Pathol, Dept Microbiol & Mol Pathol, POB 2014 Fortitude Valley, Brisbane, Qld 4006, Australia

[18] Royal Brisbane & Womens Hosp, Pathol Queensland Cent Lab, 40 Butterfield St Herston, Brisbane, Qld 4029, Australia

[19] Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, 792 Elizabeth St, Melbourne, Vic 3000, Australia

[20] Inst Pasteur, Dept Computat Biol, Evolutionary Dynam Infect Dis, 28 Rue Dr Roux, F-75015 Paris, France

来源：

VIRUS EVOLUTION | 2024年 / 10卷 / 01期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

Ross River virus; Barmah Forest virus; Alphavirus; Togaviridae; convergent evolution; discrete-trait analysis; EPIDEMIC POLYARTHRITIS; INFECTION; QUEENSLAND; MOSQUITOS; VACCINE; REPLICATION; PERFORMANCE; DATAMONKEY; DYNAMICS; HORSES;

D O I：

10.1093/ve/veae080

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ross River virus (RRV) and Barmah Forest virus (BFV) are arthritogenic arthropod-borne viruses (arboviruses) that exhibit generalist host associations and share distributions in Australia and Papua New Guinea (PNG). Using stochastic mapping and discrete-trait phylogenetic analyses, we profiled the independent evolution of RRV and BFV signature mutations. Analysis of 186 RRV and 88 BFV genomes demonstrated their viral evolution trajectories have involved repeated selection of mutations, particularly in the nonstructural protein 1 (nsP1) and envelope 3 (E3) genes suggesting convergent evolution. Convergent mutations in the nsP1 genes of RRV (residues 248 and 441) and BFV (residues 297 and 447) may be involved with catalytic enzyme mechanisms and host membrane interactions during viral RNA replication and capping. Convergent E3 mutations (RRV site 59 and BFV site 57) may be associated with enzymatic furin activity and cleavage of E3 from protein precursors assisting viral maturation and infectivity. Given their requirement to replicate in disparate insect and vertebrate hosts, convergent evolution in RRV and BFV may represent a dynamic link between their requirement to selectively 'fine-tune' intracellular host interactions and viral replicative enzymatic processes. Despite evidence of evolutionary convergence, selection pressure analyses did not reveal any RRV or BFV amino acid sites under strong positive selection and only weak positive selection for nonstructural protein sites. These findings may indicate that their alphavirus ancestors were subject to positive selection events which predisposed ongoing pervasive convergent evolution, and this largely supports continued purifying selection in RRV and BFV populations during their replication in mosquito and vertebrate hosts.

引用

页数：14