Comparison of the Effects of Inappropriate Meal Timing-Induced and Genetic Models of Circadian Clock Disruption on Uterine mRNA Expression Profiles

被引：0

作者：

Chen, Yuchen ^{[1
]}

Hosono, Takashi ^{[1
]}

Ono, Masanori ^{[2
]}

Daikoku, Takiko ^{[3
]}

Toyoda, Natsumi ^{[3
]}

Nomura, Satoshi ^{[1
]}

Kagami, Kyosuke ^{[1
]}

Orisaka, Shunsuke ^{[1
]}

Horike, Shin-ichi ^{[4
]}

Shi, Yifan ^{[5
]}

Xu, Pingping ^{[5
]}

Morishige, Jun-ichi ^{[5
]}

Fujiwara, Tomoko ^{[6
]}

Fujiwara, Hiroshi ^{[1
,7
]}

Ando, Hitoshi ^{[5
]}

机构：

[1] Kanazawa Univ, Grad Sch Med Sci, Dept Obstet & Gynecol, Kanazawa, Japan

[2] Tokyo Med Univ, Dept Obstet & Gynecol, Tokyo, Japan

[3] Kanazawa Univ, Res Ctr Expt Modeling Human Dis, Div Anim Dis Model, Kanazawa, Japan

[4] Kanazawa Univ, Res Ctr Expt Modeling Human Dis, Div Integrated Om Res, Kanazawa, Japan

[5] Kanazawa Univ, Grad Sch Med Sci, Dept Cellular & Mol Funct Anal, Kanazawa, Japan

[6] Kyoto Notre Dame Univ, Dept Human Life Environm, Kyoto, Japan

[7] OCHI YUME CLIN NAGOYA, Nagoya, Japan

来源：

JOURNAL OF NUTRITION | 2024年 / 154卷 / 12期

关键词：

circadian clock; clock genes; gene expression pro; irregular eating habits; timing of eating; uterus; CONDITIONAL DELETION; SKIPPING BREAKFAST; OBESITY; RISK; SUSCEPTIBILITY; TRANSCRIPTION; BMAL1;

D O I：

10.1016/j.tjnut.2024.10.011

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Background: Accumulating evidence reveals that inappropriate meal timing contributes to the development of lifestyle-related diseases. An underlying mechanism is thought to be the disruption of the intracellular circadian clock in various tissues based on observations in both systemic and tissue-specific clock gene-deficient mice. However, whether the effects of conditional clock gene knockout are comparable to those of inappropriate meal timing remains unclear. Objectives: This study aimed to compare the effects of a recently developed 28-h feeding cycle model with those of a core clock gene Bmal1 Methods: The models were generated by subjecting C57BL/6J mice to an 8-h/20-h feeding/fasting cycle for 2 wk and crossing Bmal1-floxed mice with PR-Cre mice. Microarray analyses were conducted using uterine samples obtained at the beginning of the dark and light periods. Results: The analyses identified 516 and 346, significantly 4-fold and 2-fold, up- or downregulated genes in the 28-h feeding cycle and Bmal1 cKO groups, respectively, compared with each control group. Among these genes, only 7 (1.4%) and 63 (18.2%) were significantly up- or downregulated in the other model. Moreover, most (n 1/4 44, 62.9%) of these genes were oppositely regulated. These findings were Conclusions: This study reveals that a 28-h feeding cycle and Bmal1 cKO differently affect gene expression profiles and highlights the need for considering this difference to assess the pathophysiology of diseases associated with inappropriate meal timing.

引用

页码：3718 / 3725

页数：8