Dental Pulp Stem Cell Lysate-Based Hydrogel Improves Diabetic Wound Healing via the Regulation of Anti-Inflammatory Macrophages and Keratinocytes

被引：0

作者：

Xu, Jianghua ^{[1
,2
,3
]}

Zhang, Changhuan ^{[4
]}

Yan, Zhuo ^{[1
,2
,3
]}

Fan, Chen ^{[1
,2
,3
]}

Yuan, Shanshan ^{[1
,2
,3
]}

Wang, Jilong ^{[1
,2
,3
]}

Zhu, Yuting ^{[1
,2
,3
]}

Luo, Lihua ^{[5
]}

Shi, Keqing ^{[1
,2
]}

Deng, Junjie ^{[1
,2
,3
]}

机构：

[1] Univ Chinese Acad Sci, Wenzhou Inst, Joint Ctr Translat Med, Wenzhou 325000, Zhejiang, Peoples R China

[2] Wenzhou Med Univ, Affiliated Hosp 1, Joint Ctr Translat Med, Wenzhou 325000, Zhejiang, Peoples R China

[3] Univ Chinese Acad Sci, Wenzhou Inst, Zhejiang Engn Res Ctr Tissue Repair Mat, Wenzhou 325000, Zhejiang, Peoples R China

[4] First Peoples Hosp Linping Dist, Hangzhou 311100, Zhejiang, Peoples R China

[5] Wenzhou Med Univ, Sch & Hosp Stomatol, Wenzhou 325035, Zhejiang, Peoples R China

来源：

ACS APPLIED BIO MATERIALS | 2024年 / 7卷 / 11期

基金：

中国国家自然科学基金;

关键词：

dental pulp stem cell lysate-based hydrogel; long-termanti-inflammation; recruiting immune cells; keratinization; diabetic wound healing; DIFFERENTIATION; PROLIFERATION; POLARIZATION; PROMOTE; PATHWAY;

D O I：

10.1021/acsabm.4c01157

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The prolonged existence of chronic wounds heightens the risk of patients experiencing chronic pain, necrosis, and amputation. Dental pulp stem cells (DPSCs) have garnered attention due to their potential immunomodulatory and tissue repair regenerative effects in the management of chronic wounds. However, stem-cell-based therapy faces challenges such as malignant differentiation, immune rejection, and long-term effectiveness. To overcome these challenges, we proposed a chronic wound therapy using a hydrogel derived from human-originated dental pulp stem cell lysate (DPSCL). Our data indicate that, with the degradation of the dental pulp stem cell lysate-based hydrogel (DPSCLH), the slowly released cell lysates recruit anti-inflammatory M2 macrophages and promote the proliferation, migration, and keratinization of HacaT cells. In addition, in vivo studies revealed that DPSCLH avoids immune rejection reactions and induces a long-term accumulation of endogenous M2 macrophages. In a mouse model of diabetic wounds, DPSCLH effectively modulates the inflammatory microenvironment around diabetic wounds, promotes the formation of the stratum corneum, and facilitates the healing of wounds, thus holding tremendous potential for the treatment of diabetic wounds.

引用

页码：7684 / 7699

页数：16