Molecular mechanism of long chain non coding RNA LINC00511 influencing breast cancer stem cells: Mechanism of VEGFR1 protein☆

A comprehensive investigation into the mechanism of VEGFR1 protein in this process was undertaken. Lentivirus-mediated RNA interference was employed to inhibit the expression of LINC00511 in breast cancer cell lines, and changes in breast cancer stem cell markers, including CD44+/CD24-, were monitored using flow cytometry. Additionally, the interaction between VEGFR1 protein and LINC00511 and the activation of its downstream signaling pathway were investigated through co-immunoprecipitation (Co-IP) and Western blot techniques. The co-immunoprecipitation experiment revealed the direct interaction between LINC00511 and VEGFR1 protein. Western blot analysis showed that after knocking down LINC00511, the phosphorylation level of VEGFR1 protein decreased, suggesting that its activity was inhibited. After treatment with the VEGFR1 inhibitor, the properties of the breast cancer stem cells were similarly inhibited, which is consistent with the effect of knocking down LINC00511.