Herein, we synthesized guanidine functionalized graphene oxide nanocomposites referred to GO-GN NCs using hummer's method. GO-GN NCs was characterized using Fourier-Transform Infrared Spectroscopy, Powder X-ray Diffraction, Thermogravimetric Analysis, Raman Spectroscopy, Field Emission Scanning Electron Microscopy, Transmission Electron Microscopy, Brunauer-Emmett-Teller (BET) surface area analysis to confirm successful formation of GO-GN NCs catalysts. The catalytic performance for GO-GN NCs was evaluated for Knoevenagel and Aldol condensation reaction in aqueous medium at ambient condition and result shows that it could be used as a highly efficient, stable, and durable catalyst. In addition, it has another feature such as easy workup procedure, large substrate tolerance, high reaction yield, good recyclability, and environmental friendliness.