Enhancing cadmium stress resilience in chickpea (Cicer arietinum L.) via exogenous melatonin application

Chickpea (Cicer arietinum L.) productivity is hindered by biotic and abiotic stresses, particularly heavy metal toxicity. The pot experiment was carried out at the botanical garden of The Islamia University of Bahawalpur, Bahawalpur-Pakistan. The experimental treatments comprised of following details: T0 = Control + 0 mu M MT, T1 = Control + 15 mu M MT, T2= Control + 30 mu M MT, T3 = 100 mu M Cd + 0 mu M MT, T4 = 100 mu M Cd + 15 mu M MT and T5 = 100 mu M Cd + 30 mu M MT. A completely randomized design (CRD) with three replicates was used. Cd stress significantly reduced shoot fresh (51.3%) and dry weight (50.4%), total chlorophyll (53.6%), and shoot Ca2+ (56.6%). However, it increased proline (38.3%), total phenolics (74.2%), glycine betaine (46.4%), TSS (67.7%), TSP (50%), SOD (49.5%), POD (107%), and CAT (74.2%). Conversely, 30 mu M MT improved shoot fresh (78.5%) and dry weight (76%), total chlorophyll (47%), SOD (26.5%), POD (35.8%), CAT (27.8%), proline (19%), TSS (24.5%), TSP (25.8%), and shoot Ca2+ (56.6%). Results indicated that MT enhanced photosynthetic pigments and antioxidant activities, maintained ion homeostasis, and reduces reactive oxygen species. Desi variety performed better than Kabuli, and 30 mu M MT application effectively mitigated Cd toxicity.