In Saccharomyces cerevisiae, Pah1 phosphatidate (PA) phosphatase, which catalyzes the Mg2+-dependent dephosphorylation of PA to produce diacylglycerol, plays a key role in utilizing PA for the synthesis of the neutral lipid triacylglycerol and thereby controlling the PA-derived membrane phospholipids. The enzyme function is controlled by its subcellular location as regulated by phosphorylation and dephosphorylation. Pah1 is initially inactivated in the cytosol through phosphorylation by multiple protein kinases and then activated via its recruitment and dephosphorylation by the protein phosphatase Nem1-Spo7 at the nuclear/endoplasmic reticulum membrane where the PA phosphatase reaction occurs. Many of the protein kinases that phosphorylate Pah1 have yet to be characterized with the identification of the target residues. Here, we established Pah1 as a bona fi de substrate of septinassociated Hsl1, a protein kinase involved in mitotic morphogenesis checkpoint signaling. The Hsl1 activity on Pah1 was dependent on reaction time and the amounts of protein kinase, Pah1, and ATP. The Hsl1 phosphorylation of Pah1 occurred on Ser-748 and Ser-773, and the phosphorylated protein exhibited a 5-fold reduction in PA phosphatase catalytic efficiency. Analysis of cells expressing the S748A and S773A mutant forms of Pah1 indicated that Hsl1-mediated phosphorylation of Pah1 promotes membrane phospholipid synthesis at the expense of triacylglycerol, and ensures the dependence of Pah1 function on the Nem1-Spo7 protein phosphatase. This work advances the understanding of how Hsl1 facilitates membrane phospholipid synthesis through the phosphorylation-mediated regulation of Pah1.
机构:
Univ Wisconsin, Genet Lab, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
MacGilvray, Matthew E.
Shishkova, Evgenia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA
Univ Wisconsin, Ctr Genom Sci Innovat, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Shishkova, Evgenia
Place, Michael
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Place, Michael
Wagner, Ellen R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA
Univ Wisconsin, Ctr Genom Sci Innovat, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Wagner, Ellen R.
Coon, Joshua J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Ctr Genom Sci Innovat, Dept Chem, Madison, WI 53706 USA
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Ctr Genom Sci Innovat, Dept Biomol Chem, Madison, WI 53706 USA
Univ Wisconsin, Morgridge Inst Res, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Coon, Joshua J.
Gasch, Audrey P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Mirheydari, Mona
Dey, Prabuddha
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Dey, Prabuddha
Stukey, Geordan J.
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Stukey, Geordan J.
Park, Yeonhee
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Park, Yeonhee
Han, Gil-Soo
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Han, Gil-Soo
Carman, George M.
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
机构:
Univ Wisconsin, Genet Lab, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
MacGilvray, Matthew E.
Shishkova, Evgenia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA
Univ Wisconsin, Ctr Genom Sci Innovat, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Shishkova, Evgenia
Place, Michael
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Place, Michael
Wagner, Ellen R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA
Univ Wisconsin, Ctr Genom Sci Innovat, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Wagner, Ellen R.
Coon, Joshua J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Ctr Genom Sci Innovat, Dept Chem, Madison, WI 53706 USA
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Ctr Genom Sci Innovat, Dept Biomol Chem, Madison, WI 53706 USA
Univ Wisconsin, Morgridge Inst Res, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Coon, Joshua J.
Gasch, Audrey P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USAUniv Wisconsin, Genet Lab, Ctr Genom Sci Innovat, Madison, WI 53706 USA
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Mirheydari, Mona
Dey, Prabuddha
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Dey, Prabuddha
Stukey, Geordan J.
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Stukey, Geordan J.
Park, Yeonhee
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Park, Yeonhee
Han, Gil-Soo
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA
Han, Gil-Soo
Carman, George M.
论文数: 0引用数: 0
h-index: 0
机构:
Rutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USARutgers State Univ, New Jersey Inst Food Nutr & Hlth, Dept Food Sci, New Brunswick, NJ 08903 USA