Simple recombinant monoclonal antibody production from Escherichia coli

被引：0
作者：
Baker, Karen [1 ]
Eastwood, Tara A. [1 ]
Garcia, Esther [2 ]
Lennon, Chris [3 ]
Mulvihill, Daniel P. [1 ]
机构：
[1] Univ Kent, Sch Biosci, Canterbury CT2 7NJ, England
[2] Rutherford Appleton Lab, Cent Laser Facil, Res Complex Harwell Sci & Technol Facil Council, Oxford OX11 0FA, England
[3] Fujifilm Diosynth Biotechnol UK Ltd, Belasis Ave, Billingham TS23 1LH, England
来源：
OPEN BIOLOGY | 2025年 / 15卷 / 02期
基金：
英国医学研究理事会;
关键词：
recombinant protein; monoclonal antibodies; bacterial expression; MUC1; IMMUNOGLOBULINS; EXPRESSION; IGG;
D O I：
10.1098/rsob.240229
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibodies are valuable biological reagents used in a wide range of discovery research, biotechnology, diagnostic and therapeutic applications. Currently, both commercial and laboratory-scale antibody production is reliant on expression from mammalian cells, which can be time-consuming and requires the use of specialist facilities and costly growth reagents. Here, we describe a simple, rapid and cheap method for producing and isolating functional monoclonal antibodies and antibody fragments from bacterial cells that can be used in a range of laboratory applications. This simple method only requires access to basic microbial cell culture and molecular biology equipment, making scalable in-house antibody production accessible to the global diagnostics, therapeutics and molecular bioscience research communities.
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页数：8
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