Evidence for secondary ciliary dyskinesia in patients with cystic fibrosis

被引:1
作者
Bonhiver, Romane [1 ,2 ]
Bricmont, Noemie [1 ,2 ]
Pirotte, Maud [1 ]
Wuidart, Marc-Antoine [3 ]
Monseur, Justine [4 ]
Benchimol, Lionel [5 ]
Poirrier, Anne-Lise [5 ]
Moermans, Catherine [1 ,6 ]
Calmes, Doriane [6 ]
Schleich, Florence [1 ,6 ]
Louis, Renaud [1 ,6 ]
Seghaye, Marie-Christine [7 ,8 ]
Kempeneers, Celine [1 ,2 ]
机构
[1] Univ Liege, GIGA Res Ctr, Pneumol Lab, Grp I3, Liege, Belgium
[2] Univ Hosp Liege, Dept Pediat, Div Respirol, Liege, Belgium
[3] Reg Hosp Ctr Liege, Div Physiotherapy, Liege, Belgium
[4] Univ Liege, Biostat & Res Method Ctr, Publ Hlth Dept, Liege, Belgium
[5] Univ Hosp Liege, Dept ENT, Liege, Belgium
[6] Univ Hosp Liege, Dept Pneumol, Liege, Belgium
[7] Univ Hosp Liege, Dept Pediat, Div Cardiol, Liege, Belgium
[8] Univ Liege, Liege, Belgium
关键词
Cilia; Ciliary dyskinesia; Cystic fibrosis; Mucociliary clearance; BEAT FREQUENCY; MUCOCILIARY CLEARANCE; ULTRASTRUCTURE; INFLAMMATION;
D O I
10.1016/j.jcf.2024.10.003
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: Mucociliary clearance (MCC) impairment can be due to mucus abnormalities or to a ciliary dysfunction, which can be innate, or secondary to infection and/or inflammation. In cystic fibrosis (CF), it is well documented that MCC is impaired due to mucus abnormalities, but little is known concerning ciliary beating. This study aimed to confirm that ciliary dyskinesia is present in CF, and if this might be innate or secondary to the chronic infection and/or inflammation. Methods: Ciliated epithelial samples were obtained by nasal brushing from 51 CF patients, and from 30 healthy subjects. Ciliary beating was evaluated using digital high-speed videomicroscopy at 37 degrees C, allowing to evaluate ciliary beat frequency (CBF) and the percentage of abnormal beat pattern (CBP); this was repeated after air-liquid interface (ALI) cell culture. Results: Ciliary dyskinesia was higher in CF patients than in healthy subjects, with a lower CBF and a higher percentage of abnormal CBP. Ciliary dyskinesia, already present in childhood, normalized after ALI cell culture. A chronic airway colonization did not worsen ciliary dyskinesia. Conclusions: We showed that, in CF, a ciliary dyskinesia, present from childhood, might contribute to the impaired MCC. Our results also found that the abnormal ciliary beating was not associated with a chronic infection, and resolved after ALI cell culture, suggesting that ciliary dyskinesia in CF is not innate, and might be secondary to chronic inflammation.
引用
收藏
页码:193 / 200
页数:8
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