Interstitial microbial communities of coastal sediments are dominated by Nanoarchaeota

被引:0
作者
Brick, Simone [1 ]
Niggemann, Jutta [1 ]
Reckhardt, Anja [1 ]
Koenneke, Martin [1 ]
Engelen, Bert [1 ]
机构
[1] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm ICBM, Sch Math & Sci, Oldenburg, Germany
关键词
subterranean estuary (STE); high-energy beach; epipsammon; interstitial communities; DPANN superphylum; Nanoarchaeota; candidate phyla radiation; TIDAL-FLAT SEDIMENTS; ADVECTIVE TRANSPORT; ORGANIC-MATTER; WATER; BACTERIA; BIOGEOCHEMISTRY; SUBSURFACE; CARBON; DYNAMICS; NUTRIENT;
D O I
10.3389/fmicb.2025.1532193
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microbial communities in subsurface coastal sediments are highly diverse and play an important role in nutrient cycling. While the major fraction of microorganisms in sandy sediments lives as epipsammon (attached to sand grains), only a small fraction thrives in the interstitial porewaters. So far, little is known about the composition of these free-living microbial communities. In the subsurface of the sandy beach, investigated in this study, we compared the archaeal and bacterial community structures within sediments and corresponding porewaters applying 16S rRNA gene sequencing. We found that the free-living prokaryotes only had a proportion of about 0.2-2.3% of the bulk communities, depending on the pore space. The interstitial microbial communities showed a small overlap with the attached fraction of 4-7% ASVs, and comprised a unique composition of 75-81% ASVs found exclusively in the porewaters. They were more diverse than the respective sediment-attached fraction and showed a much higher archaea-to-bacteria ratio. The archaea were mainly affiliated to Nanoarchaeota of the DPANN superphylum, with a relative abundance up to 50% of the interstitial communities. The bacterial fraction included several species related to the Candidate Phyla Radiation (CPR). Both prokaryotic lineages are known to have small cell sizes, comprising not-yet cultured species with unidentified metabolic functions. Our findings were supported by the investigation of an adjacent tidal flat, showing a similar trend. Thus, our results indicate the presence of distinct interstitial microbial communities in the subsurface of coastal sediments. This natural enrichment of not-yet cultured Nanoarchaeota and members of the CPR provides the opportunity for targeted metagenomic analyses or even isolating members of these groups for further metabolic characterization.
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页数:12
相关论文
共 71 条
[1]  
Abarike GA, 2024, FRONT MAR SCI, V11, DOI 10.3389/fmars.2024.1501781
[2]   The effect of sediment grain properties and porewater flow on microbial abundance and respiration in permeable sediments [J].
Ahmerkamp, Soeren ;
Marchant, Hannah K. ;
Peng, Chao ;
Probandt, David ;
Littmann, Sten ;
Kuypers, Marcel M. M. ;
Holtappels, Moritz .
SCIENTIFIC REPORTS, 2020, 10 (01)
[3]   Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system [J].
Anantharaman, Karthik ;
Brown, Christopher T. ;
Hug, Laura A. ;
Sharon, Itai ;
Castelle, Cindy J. ;
Probst, Alexander J. ;
Thomas, Brian C. ;
Singh, Andrea ;
Wilkins, Michael J. ;
Karaoz, Ulas ;
Brodie, Eoin L. ;
Williams, Kenneth H. ;
Hubbard, Susan S. ;
Banfield, Jillian F. .
NATURE COMMUNICATIONS, 2016, 7
[4]  
[Anonymous], 2013, 16S metagenomic sequencing library preparation, P1
[5]   Short-term intertidal bar mobility on a ridge-and-runnel beach, Merlimont, northern France [J].
Anthony, EJ ;
Levoy, F ;
Monfort, O ;
Degryse-Kulkarni, C .
EARTH SURFACE PROCESSES AND LANDFORMS, 2005, 30 (01) :81-93
[6]   The Beach Aquifer Microbiome: Research Gaps and Data Needs [J].
Archana, Anand ;
Francis, Christopher A. ;
Boehm, Alexandria B. .
FRONTIERS IN ENVIRONMENTAL SCIENCE, 2021, 9
[7]   Spatial and seasonal variations of sulphate, dissolved organic carbon, and nutrients in deep pore waters of intertidal flat sediments [J].
Beck, Melanie ;
Dellwig, Olaf ;
Liebezeit, Gerd ;
Schnetger, Bernhard ;
Brumsack, Hans-Juergen .
ESTUARINE COASTAL AND SHELF SCIENCE, 2008, 79 (02) :307-316
[8]   The drivers of biogeochemistry in beach ecosystems: A cross-shore transect from the dunes to the low-water line [J].
Beck, Melanie ;
Reckhardt, Anja ;
Amelsberg, Julia ;
Bartholoma, Alexander ;
Brumsack, Hans-Juergen ;
Cypionka, Heribert ;
Dittmar, Thorsten ;
Engelen, Bert ;
Greskowiak, Janek ;
Hillebrand, Helmut ;
Holtappels, Moritz ;
Neuholz, Rene ;
Koester, Juergen ;
Kuypers, Marcel M. M. ;
Massmann, Gudrun ;
Meier, Daniela ;
Niggemann, Jutta ;
Paffrath, Ronja ;
Pahnke, Katharina ;
Rovo, Sandra ;
Striebel, Maren ;
Vandieken, Verona ;
Wehrmann, Achim ;
Zielinski, Oliver .
MARINE CHEMISTRY, 2017, 190 :35-50
[9]   Deep pore water profiles reflect enhanced microbial activity towards tidal flat margins [J].
Beck, Melanie ;
Koester, Juergen ;
Engelen, Bert ;
Holstein, Jan M. ;
Gittel, Antje ;
Koenneke, Martin ;
Riedel, Thomas ;
Wirtz, Kai ;
Cypionka, Heribert ;
Rullkoetter, Juergen ;
Brumsack, Hans-Juergen .
OCEAN DYNAMICS, 2009, 59 (02) :371-383
[10]   Groundwater and pore water inputs to the coastal zone [J].
Burnett, WC ;
Bokuniewicz, H ;
Huettel, M ;
Moore, WS ;
Taniguchi, M .
BIOGEOCHEMISTRY, 2003, 66 (1-2) :3-33