PBAN regulates sex pheromone biosynthesis by Ca2+/CaN/ACC and Ca2+/PKC/HK2 signal pathways in Spodoptera litura

被引：1

作者：

Zhang, Yao ^{[1
]}

He, Ruolan ^{[1
]}

She, Zelong ^{[1
]}

Yin, Xinming ^{[1
]}

Li, Xiang ^{[1
]}

Yao, Shuangyan ^{[1
]}

Du, Mengfang ^{[1
]}

An, Shiheng ^{[1
]}

机构：

[1] Henan Agr Univ, Coll Plant Protect, State Key Lab Wheat & Maize Crop Sci, Henan Int Lab Green Pest Control, Zhengzhou, Peoples R China

来源：

INSECT MOLECULAR BIOLOGY | 2025年 / 34卷 / 02期

基金：

中国国家自然科学基金;

关键词：

acetyl-CoA carboxylase; Calcineurin; hexokinase2; sex pheromone; Spodoptera litura; FIELD EVOLVED RESISTANCE; ACTIVATING NEUROPEPTIDE; DIAPAUSE HORMONE; MOLECULAR CHARACTERIZATION; CHEMISTRY INSECTICIDES; SUBESOPHAGEAL GANGLION; HELIOTHIS-VIRESCENS; PRECURSOR PROTEIN; HELICOVERPA-ZEA; LEPIDOPTERA;

D O I：

10.1111/imb.12976

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sex pheromones emitted by female moths play important roles in mate attraction. The molecular mechanism underlying pheromone biosynthesis activating neuropeptide (PBAN)-regulated sex pheromone biosynthesis has been well elucidated in many moth species, although this mechanism is species-dependent. Spodoptera litura, an important pest, has caused serious economic losses to agricultural production, yet the mechanism for its sex pheromone biosynthesis has not been fully identified. The present study investigates in detail mechanism underlying PBAN-regulated sex pheromone biosynthesis in S. litura. The transcriptome sequencing of S. litura pheromone glands (PGs) was analysed to identify a serial of candidate genes potentially involved in sex pheromone biosynthesis. Further investigation revealed a bimodal pattern in both sex pheromone release and mating frequency. PBAN was found to regulate sex pheromone biosynthesis via its receptor by using Ca2+ as a secondary messenger, as demonstrated by RNA interference and the application of pharmacological inhibitors. Furthermore, PBAN/Ca2+ signalling activated calcineurin (CaN) and acetyl-CoA carboxylase (ACC), which mediated sex pheromone biosynthesis in response to PBAN stimulation. Mostly importantly, hexokinase 2 (HK2) was confirmed to be activated by PBAN/PBANR /Ca2+/PKC signalling via phosphorylation at two specific sites (ser423 and ser434 sites of HK2). Overall, our findings shed light on the intricate processes involved in sex pheromone production in S. litura, in which PBAN regulates sex pheromone biosynthesis through PBAN/PBANR/Ca2+/CaN/ACC and PBAN/PBANR/Ca2+/PKC/HK2 signalling pathways. These insights significantly contribute to our comprehension of the specific mechanisms underlying sex pheromone biosynthesis in this moth species.

引用

页码：347 / 362

页数：16

共 57 条

[1] Evidence for field evolved resistance to newer insecticides in Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan [J].

Ahmad, Munir ;

Sayyed, Ali H. ;

Saleem, Mushtaq A. ;

Ahmad, Mushtaq .

CROP PROTECTION, 2008, 27 (10) :1367-1372

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3]

Bateman A, 2002, NUCLEIC ACIDS RES, V30, P276, DOI [10.1093/nar/gkr1065, 10.1093/nar/gkp985, 10.1093/nar/gkh121]

[4] Hexokinase Is Required for Sex Pheromone Biosynthesis in Helicoverpa armigera [J].

Chang, Yanpeng ;

Zhang, Yunhui ;

Geng, Zichen ;

Yao, Shuangyan ;

Zhao, Wenli ;

Yin, Xinming ;

An, Shiheng .

INSECTS, 2021, 12 (10)

[5] Rice spikelet formation inhibition caused by decreased sugar utilization under high temperature is associated with brassinolide decomposition [J].

Chen, Yanhua ;

Chen, Huizhe ;

Xiang, Jing ;

Zhang, Yikai ;

Wang, Zhigang ;

Zhu, Defeng ;

Wang, Junke ;

Zhang, Yuping ;

Wang, Yaliang .

ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2021, 190

[6] MOLECULAR-CLONING OF THE PHEROMONE BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE IN HELICOVERPA-ZEA [J].

DAVIS, MTB ;

VAKHARIA, VN ;

HENRY, J ;

KEMPE, TG ;

RAINA, AK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :142-146

[7] A hormone from the uterus of the tsetse fly, Glossina morsitans, stimulates parturition and abortion [J].

Denlinger, DL ;

Zdarek, J .

JOURNAL OF INSECT PHYSIOLOGY, 1997, 43 (02) :135-142

[8] Target and non-target response of Swietenia Mahagoni Jacq. chemical constituents against tobacco cutworm Spodoptera litura Fab. and earthworm, Eudrilus eugeniae Kinb [J].

Dinesh-Kumar, Anandan ;

Srimaan, Elangovan ;

Chellappandian, Muthiah ;

Vasantha-Srinivasan, Prabhakaran ;

Karthi, Sengodan ;

Thanigaivel, Annamalai ;

Ponsankar, Athirstam ;

Chanthini, Kanagaraj Muthu-Pandian ;

Shyam-Sundar, Narayanan ;

Annamalai, Mahendiran ;

Kalaivani, Kandaswamy ;

Hunter, Wayne B. ;

Senthil-Nathan, Sengottayan .

CHEMOSPHERE, 2018, 199 :35-43

[9]

Du MF, 2017, MOL CELL PROTEOMICS, V16, P2138, DOI [10.1074/mcp.RA117.000065, 10.1074/mcp.ra117.000065]

[10] Phosphorylation of Yeast Hexokinase 2 Regulates Its Nucleocytoplasmic Shuttling [J].

Fernandez-Garcia, Paula ;

Pelaez, Rafael ;

Herrero, Pilar ;

Moreno, Fernando .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (50) :42151-42164

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