HIV-1 assembly - when virology meets biophysics

被引:2
作者
Lacouture, Claire [1 ]
Carrio, Baptiste [1 ]
Favard, Cyril [1 ]
Muriaux, Delphine [1 ]
机构
[1] Montpellier Univ, Inst Rech Infectiol Montpellier IRIM, Membrane Domains & Viral Assembly, UMR 9004,CNRS, 1919 Route Mende, F-34293 Montpellier 5, France
关键词
KEY WORDS; HIV-1; Gag; Membrane curvature; Phospholipids; Cortical actin; HUMAN-IMMUNODEFICIENCY-VIRUS; PLASMA-MEMBRANE; GAG PROTEIN; TYPE-1; GAG; NUCLEOCAPSID DOMAIN; PARTICLE RELEASE; PHASE-SEPARATION; STRUCTURAL BASIS; LIPID-BILAYERS; CELL-MEMBRANE;
D O I
10.1242/jcs.262064
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cells naturally produce vesicles that bud from different lipid membranes using dedicated molecular machineries. Enveloped RNA viruses, including human immunodeficiency virus type 1 (HIV-1), also generate particles that bud from host cell membranes by hijacking cellular factors and signaling pathways similar to those involved in the budding of extracellular vesicles. HIV-1 buds from the host cell plasma membrane mainly via the self-assembly of Gag, a structural protein. Gag is a polyprotein that forms assembly complexes containing viral genomic RNA (gRNA), host cell lipids and proteins. HIV-1 Gag binds and segregates host cell plasma membrane lipids while self-assembling simultaneously on the gRNA and the plasma membrane. This self-assembly causes membrane bending and formation of a new viral particle with the help of host cell proteins, likely including cortical actin-associated factors. However, it is unclear whether the energy of Gag self-assembly is sufficient to generate new HIV-1 particles. In this Review, we discuss these processes in the light of the past and recent virology literature, incorporating lessons from studies on the quantitative biophysics of viral self-assembly, and explore how Gag might reorganize the plasma membrane and divert host cell membrane curving proteins and cortical actin-related factors to achieve particle assembly and budding.
引用
收藏
页数:13
相关论文
共 50 条
[41]   Role of the HIV-1 matrix protein in Gag intracellular trafficking and targeting to the plasma membrane for virus assembly [J].
Ghanam, Ruba H. ;
Samal, Alexandra B. ;
Fernandez, Timothy F. ;
Saad, Jamil S. .
FRONTIERS IN MICROBIOLOGY, 2012, 3
[42]   RRE-dependent HIV-1 Env RNA effects on Gag protein expression, assembly and release [J].
Lopez, Claudia S. ;
Sloan, Rachel ;
Cylinder, Isabel ;
Kozak, Susan L. ;
Kabat, David ;
Barklis, Eric .
VIROLOGY, 2014, 462 :126-134
[43]   Role of Gag and lipids during HIV-1 assembly in CD4+ T cells and macrophages [J].
Mariani, Charlotte ;
Desdouits, Marion ;
Favard, Cyril ;
Benaroch, Philippe ;
Muriaux, Delphine M. .
FRONTIERS IN MICROBIOLOGY, 2014, 5
[44]   Identifying the assembly intermediate in which Gag first associates with unspliced HIV-1 RNA suggests a novel model for HIV-1 RNA packaging [J].
Barajas, Brook C. ;
Tanaka, Motoko ;
Robinson, Bridget A. ;
Phuong, Daryl J. ;
Chutiraka, Kasana ;
Reed, Jonathan C. ;
Lingappa, Jaisri R. .
PLOS PATHOGENS, 2018, 14 (04)
[45]   Fluorescence Microscopy of the HIV-1 Envelope [J].
Carravilla, Pablo ;
Nieva, Jose L. ;
Eggeling, Christian .
VIRUSES-BASEL, 2020, 12 (03)
[46]   Morphogenesis of the infectious HIV-1 virion [J].
Sakuragi, Jun-ichi .
FRONTIERS IN MICROBIOLOGY, 2011, 2
[47]   Functional Redundancy in HIV-1 Viral Particle Assembly [J].
O'Carroll, Ina P. ;
Crist, Rachael M. ;
Mirro, Jane ;
Harvin, Demetria ;
Soheilian, Ferri ;
Kamata, Anne ;
Nagashima, Kunio ;
Rein, Alan .
JOURNAL OF VIROLOGY, 2012, 86 (23) :12991-12996
[48]   HIV-1 Mutant Assembly, Processing and Infectivity Expresses Pol Independent of Gag [J].
Yu, Fu-Hsien ;
Huang, Kuo-Jung ;
Wang, Chin-Tien .
VIRUSES-BASEL, 2020, 12 (01)
[49]   Challenges in Virological Diagnosis of HIV-1 Transmission from Sexual Abuse HIV-1 Genetic Links are Mandatory [J].
Ehrnst, Anneka .
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2013, 69 :116-121
[50]   Role of HIV-1 nucleocapsid protein in HIV-1 reverse transcription [J].
Levin, Judith G. ;
Mitra, Mithun ;
Mascarenhas, Anjali ;
Musier-Forsyth, Karin .
RNA BIOLOGY, 2010, 7 (06) :754-774