CARD9 protein SUMOylation regulates HOXB5 nuclear translocation and Parkin-mediated mitophagy in myocardial I/R injury

被引：0

作者：

Li, Yuanbin ^{[1
]}

Tang, Yuting ^{[2
]}

Yan, Xu ^{[3
]}

Lin, Hui ^{[1
]}

Jiang, Wanjin ^{[1
]}

Zhang, Luwei ^{[1
]}

Zhao, Hu ^{[3
]}

Chen, Zhuang ^{[1
]}

机构：

[1] Hunan Tradit Chinese Med Coll, Dept Med, Zhuzhou 412012, Hunan, Peoples R China

[2] Cent South Univ, Affiliated Canc Hosp, Hunan Canc Hosp, Xiangya Sch Med,Dept Pathol, Changsha, Hunan, Peoples R China

[3] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept Cardiovasc, Changsha, Hunan, Peoples R China

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2024年 / 28卷 / 21期

基金：

中国国家自然科学基金;

关键词：

CARD9; HOXB5; nuclear; Mitophagy; O-GlcNAc glycosylation; SUMOylation; translocation; ACTIVATION; APOPTOSIS; PROLIFERATION; CELL;

D O I：

10.1111/jcmm.70195

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Myocardial injury induced by ischemia-reperfusion (I/R) remains a difficult clinical problem. However, the exact mechanisms underlying I/R-induced have yet to be clarified. CARD9 is an important cytoplasmic-binding protein. In this study, an immunocoprecipitation assay showed that SUMOylation of the CARD9 protein promoted the binding of CARD9 to HOXB5, but hindered the O-GlcNAc glycosylation of HOXB5, a predicted transcription factor of Parkin and a key factor in mitophagy. O-GlcNAc glycosylation is an important signal for translocation of proteins from the cytoplasm to the nucleus. CARD9 protein SUMOylation is regulated by PIAS3, which is related to I/R-induced myocardial injury. Therefore, we propose that knockdown of PIAS3 inhibits SUMOylation of the CARD9 protein, facilitates the dissociation of CARD9 and HOXB5, which increases the O-GlcNAc-mediated glycosylation of HOXB5, while the resulting HOXB5 nuclear translocation promotes Parkin-induced mitophagy and alleviates myocardial I/R injury.

引用

页数：15