Lateral flow immunoassay using plasmonic scattering

被引:0
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作者
Bobin Lee [1 ]
Byungho Park [1 ]
Daeun Kim [1 ]
Chaewon Jung [2 ]
Jun Hyeok Park [3 ]
Ji-Ho Park [3 ]
Young Eun Lee [3 ]
Myung Geun Shin [4 ]
Min-Gon Kim [4 ]
Nan Ei Yu [3 ]
Joon Heon Kim [1 ]
Kihyeun Kim [1 ]
机构
[1] Gwangju Institute of Science and Technology (GIST),Advanced Photonics Research Institute (APRI)
[2] Chonnam National University,Department of Biotechnology and Bioengineering
[3] Gwangju Institute of Science and Technology (GIST),Department of Chemistry
[4] Chonnam National University Medical School and Chonnam National University Hwasun Hospital,Department of Laboratory Medicine
[5] ON NANOBIOLAB Inc.,undefined
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D O I
10.1038/s41467-025-58663-z
中图分类号
学科分类号
摘要
The lateral flow immunoassay (LFIA) is one of the most successful sensing platforms for real-world point-of-care (POC) testing. However, achieving PCR-level sensitivity without compromising the inherent advantages of LFIA, such as rapid and robust operation, affordability, and naked-eye detection, has remained a primary challenge. In this study, a plasmonic scattering-utilising LFIA was proposed, created by transparentising a nitrocellulose membrane and placing a light-absorbing backing card under the membrane. This LFIA minimised the background signal from its matrix, leading to substantially enhanced sensitivity and enabling naked-eye detection of the plasmonic scattering signal from gold nanoparticles without optics. Our plasmonic scattering-utilising LFIA showed an approximately 2600–4400 times higher detection limit compared with that of commercial LFIAs in influenza A assays. In addition, it exhibited 90% sensitivity in clinical validation, approaching PCR-level sensitivity, while commercial LFIAs showed 23–30% sensitivity. The plasmonic scattering-utilising LFIA plays a ground-breaking role in POC diagnostics and significantly boosts follow-up research.
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