Neutrophil Elastase Targets Select Proteins on Human Blood-Monocyte-Derived Macrophage Cell Surfaces

被引：1

作者：

Ahmed, Nadia Tasnim ^{[1
]}

Kummarapurugu, Apparao B. ^{[2
]}

Zheng, Shuo ^{[2
]}

Bulut, Gamze ^{[2
]}

Kang, Le ^{[3
]}

Batheja, Aashish ^{[4
]}

Hawkridge, Adam ^{[1
]}

Voynow, Judith A. ^{[2
]}

机构：

[1] Virginia Commonwealth Univ VCU, Sch Pharm, Richmond, VA 23298 USA

[2] VCU, Childrens Hosp Richmond, Richmond, VA 23298 USA

[3] Virginia Commonwealth Univ VCU, Dept Biostat, Richmond, VA 23298 USA

[4] Virginia Commonwealth Univ VCU, Sch Med, Richmond, VA 23298 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 23期

关键词：

neutrophil elastase; ELANE; cystic fibrosis; COPD; macrophages; polarization; inflammation; proteoglycans; HUMAN-LEUKOCYTE ELASTASE; CYSTIC-FIBROSIS; NEUROPILIN-1; RECEPTOR; CD99; PROTEOGLYCANS; GLYPICAN-4; EXPRESSION; CLEAVAGE;

D O I：

10.3390/ijms252313038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Neutrophil elastase (NE) has been reported to be a pro-inflammatory stimulus for macrophages. The aim of the present study was to determine the impact of NE exposure on the human macrophage proteome and evaluate its impact on pro-inflammatory signals. Human blood monocytes from healthy volunteers were differentiated to macrophages and then exposed to either 500 nM of NE or control vehicle for 2 h in triplicate. Label-free quantitative proteomics analysis identified 41 differentially expressed proteins in the NE versus control vehicle datasets. A total of 26 proteins were downregulated and of those, 21 were cell surface proteins. Importantly, four of the cell surface proteins were proteoglycans: neuropilin 1 (NRP1), syndecan 2 (SDC2), glypican 4 (GPC4), and CD99 antigen-like protein 2 (CD99L2) along with neuropilin 2 (NRP2), CD99 antigen (CD99), and endoglin (ENG) which are known interactors. Additional NE-targeted proteins related to macrophage function were also measured including CD40, CD48, SPINT1, ST14, and MSR1. Collectively, this study provides a comprehensive unbiased view of selective NE-targeted cell surface proteins in chronically inflamed lungs.

引用

页数：14