Freshwater ecosystems face increasing pressures from human activities, leading to degraded water quality and altered habitats for aquatic species. This study investigates the relationship between water quality and waterbird distribution along the Lieve River, Belgium, based on manually conducted waterbird counts and water quality data collected from 48 transects in March 2024. Localized eutrophication was evident, with TN (2.7-5.6 mg L-1), TP (up to 0.46 mg L-1), and chlorophyll-a (median 70 ppb) exceeding environmental thresholds. Prati index analysis revealed that 58.3% of the sampling points along the Lieve River were categorized as "polluted", reflecting extensive water quality degradation. Eurasian coots (71.4%) and wild ducks (72.4%) were predominantly found in polluted areas, thriving in nutrient-enriched habitats linked to high TP levels. In contrast, common moorhens (80.3%) preferred acceptable quality areas, indicating higher water quality requirements. These findings indicate that phosphate is a key driver of waterbody eutrophication, as evidenced by the TP concentrations measured on-site, which far exceed the thresholds set by environmental standards. Future research should explore advanced monitoring approaches to improve waterbird and water quality assessments, ensuring the conservation of the Lieve River as one of Europe's oldest artificial canals, and the protection of its waterbird habitats.