Exosome-like Nanoparticles, High in Trans-δ-Viniferin Derivatives, Produced from Grape Cell Cultures: Preparation, Characterization, and Anticancer Properties

被引：3

作者：

Shkryl, Yury ^{[1
]}

Tsydeneshieva, Zhargalma ^{[1
,2
]}

Menchinskaya, Ekaterina ^{[3
]}

Rusapetova, Tatiana ^{[1
]}

Grishchenko, Olga ^{[1
]}

Mironova, Anastasia ^{[1
]}

Bulgakov, Dmitry ^{[1
]}

Gorpenchenko, Tatiana ^{[1
]}

Kazarin, Vitaly ^{[1
]}

Tchernoded, Galina ^{[1
]}

Bulgakov, Victor ^{[1
]}

Aminin, Dmitry ^{[3
]}

Yugay, Yulia ^{[1
]}

机构：

[1] Russian Acad Sci, Fed Sci Ctr East Asia Terr Biodivers, Far Eastern Branch, Vladivostok 690022, Russia

[2] Far Eastern Fed Univ, Inst Biotechnol Bioengn & Food Syst, Adv Engn Sch, Vladivostok 690922, Russia

[3] Russian Acad Sci, GB Elyakov Pacific Inst Bioorgan Chem, Far Eastern Branch, Vladivostok 690022, Russia

来源：

BIOMEDICINES | 2024年 / 12卷 / 09期

关键词：

extracellular vesicles; cancer; callus culture; salicylic acid; Vitis vinifera; PLANT EXTRACELLULAR VESICLES; DRUG-DELIVERY; NANOVESICLES; INHIBIT; CANCER; PHOSPHATIDYLSERINE; COMMUNICATION; REVEALS; DEFINES; GROWTH;

D O I：

10.3390/biomedicines12092142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Recent interest in plant-derived exosome-like nanoparticles (ENs) has surged due to their therapeutic potential, which includes antioxidant, anti-inflammatory, and anticancer activities. These properties are attributed to their cargo of bioactive metabolites and other endogenous molecules. However, the properties of ENs isolated from plant cell cultures remain less explored. Methods: In this investigation, grape callus-derived ENs (GCENs) were isolated using differential ultracentrifugation techniques. Structural analysis through electron microscopy, nanoparticle tracking analysis, and western blotting confirmed that GCENs qualify as exosome-like nanovesicles. Results: These GCENs contained significant amounts of microRNAs and proteins characteristic of plant-derived ENs, as well as trans-delta-viniferin, a notable stilbenoid known for its health-promoting properties. Functional assays revealed that the GCENs reduced the viability of the triple-negative breast cancer cell line MDA-MB-231 in a dose-dependent manner. Moreover, the GCENs exhibited negligible effects on the viability of normal human embryonic kidney (HEK) 293 cells, indicating selective cytotoxicity. Notably, treatment with these GCENs led to cell cycle arrest in the G1 phase and triggered apoptosis in the MDA-MB-231 cell line. Conclusions: Overall, this study underscores the potential of grape callus-derived nanovectors as natural carriers of stilbenoids and proposes their application as a novel and effective approach in the management of cancer.

引用

页数：25

共 116 条

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