The immune landscape of murine skeletal muscle regeneration and aging

被引：4

作者：

Sousa, Neuza S. ^{[1
,2
]}

Bica, Marta ^{[1
,2
]}

Bras, Margarida F. ^{[1
]}

Sousa, Ana C. ^{[1
]}

Antunes, Ines B. ^{[1
]}

Encarnacao, Isabel A. ^{[1
]}

Costa, Tiago M. ^{[1
,2
]}

Martins, Ines B. ^{[1
,2
]}

Barbosa-Morais, Nuno L. ^{[1
]}

Sousa-Victor, Pedro ^{[1
]}

Neves, Joana ^{[1
]}

机构：

[1] GIMM Gulbenkian Inst Mol Med, P-1649035 Lisbon, Portugal

[2] Univ Lisbon, Fac Med, P-1649028 Lisbon, Portugal

来源：

CELL REPORTS | 2024年 / 43卷 / 11期

关键词：

Highlights; skeletal muscle; DENDRITIC CELLS; T-CELLS; MACROPHAGES; REJUVENATION; EXPRESSION; ACCUMULATION; INFLAMMATION; PROGENITORS; GROWTH;

D O I：

10.1016/j.celrep.2024.114975

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Age-related alterations in the immune system are starting to emerge as key contributors to impairments found in aged organs. A decline in regenerative capacity is a hallmark of tissue aging; however, the contribution of immune aging to regenerative failure is just starting to be explored. Here, we apply a strategy combining single-cell RNA sequencing with flow cytometry, histological analysis, and functional assays to perform a complete analysis of the immune environment of the aged regenerating skeletal muscle on a time course following injury with single-cell resolution. Our results reveal an unanticipated complexity and functional heterogeneity in immune populations within the skeletal muscle that have been regarded as homogeneous. Furthermore, we uncover a profound remodeling of both myeloid and lymphoid compartments in aging. These discoveries challenge established notions on immune regulation of skeletal muscle regeneration, providing a set of potential targets to improve skeletal muscle health and regenerative capacity in aging.

引用

页数：25

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