Gelation and Cryogelation of Chitosan: Origin of Low Efficiency of Diglycidyl Ethers as Cross-Linkers in Acetic Acid Solutions

被引：0

作者：

Privar, Yuliya ^{[1
]}

Skatova, Anna ^{[1
]}

Golikov, Alexey ^{[1
]}

Boroda, Andrey ^{[2
]}

Bratskaya, Svetlana ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem, Far Eastern Branch, 159,Prosp 100 Letiya Vladivostoka, Vladivostok 690022, Russia

[2] Russian Acad Sci, AV Zhirmunsky Natl Sci Ctr Marine Biol, Far Eastern Branch, 17,Palchevskogo St, Vladivostok 690041, Russia

来源：

POLYSACCHARIDES | 2024年 / 5卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

chitosan; cross-linking; grafting; diglycidyl ethers; gelation time; compressive strength; SCAFFOLDS;

D O I：

10.3390/polysaccharides5040046

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Although diglycidyl ethers of glycols (DEs)-FDA-approved reagents for biomedical applications-were considered unsuitable for the fabrication of chitosan (CH) hydrogels and cryogels, we have recently shown that CH cross-linking with DEs is possible, but its efficiency depends on the nature of the acid used to dissolve chitosan and pH. To elucidate the origin of the low efficiency of chitosan interactions with DEs in acetic acid solutions, we have put forward two hypotheses: (i) DEs are consumed in a side reaction with acetic acid; (ii) DE chain length strongly affects the probability of cross-linking. We then verified them using FT-IR spectroscopy, rheological measurements, and uniaxial compression tests. The formation of esters in acetic acid solutions was confirmed for ethylene glycol diglycidyl ether (EGDE) and poly(ethylene glycol) diglycidyl ether (PEGDE). By the 7th day of gelation at pH 5.5, the G'HCl/G'HAc ratio was 5.1 and 1.5 for EGDE and PEGDE, respectively, indicating that the loss of cross-linking efficiency in acetic acid solution was less pronounced for the long-chain cross-linker. Under conditions of cryotropic gelation, only weak cryogels were obtained from acetic acid solutions at a DE:CH molar ratio of 1:1, while stable cryogels were fabricated at a molar ratio of 1:20 from HCl solutions.

引用

页码：731 / 742

页数：12

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