Rennet-assisted modification for enhanced freeze-thaw stability in sodium caseinate-stabilized high internal phase emulsions

被引:1
作者
Zhang, Ling [1 ]
Xiao, Feng [1 ]
Hu, Sijie [1 ]
Bi, Anqi [1 ]
Du, Ming [1 ]
Xu, Xianbing [1 ]
机构
[1] Dalian Polytech Univ, Natl Engn Res Ctr Seafood, Sch Food Sci & Technol, SKL Marine Food Proc & Safety Control, Dalian 116034, Peoples R China
关键词
Freeze-thaw stability; Emulsions; Sodium caseinate; Rennet; SOY;
D O I
10.1016/j.foodchem.2025.143599
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Poor freeze-thaw stability seriously limits the application of Pickering emulsions in the frozen food industry. This study developed sodium caseinate-stabilized high internal phase emulsions (NaCas-HIPEs) with enhanced freezethaw stability through rennet modification. The freeze-thaw stability and 3D printing properties of NaCas-HIPEs significantly improved as rennet addition increased from 0 % to 0.5 (w/v). NaCas-HIPEs with 0.5 % rennet even maintained a stable oil-in-water emulsion structure after five freeze-thaw cycles. Changes in enthalpy and freezing/thawing point demonstrated that rennet modification improved freeze-thaw stability by reducing the ice crystal formation. Additionally, increasing rennet concentration significantly enhanced the apparent viscosity and viscoelasticity of NaCas-HIPEs, restricting ice crystal growth and preventing droplet aggregation during freezing and thawing. This improvement is attributed to the strong gel networks formed by rennet-induced casein between droplets, as shown by the cryo-SEM microscopy and SDS-PAGE analysis. This study presents an effective method for producing freeze-thaw stable emulsions, offering promising applications in the rapidly growing ready-to-eat food industry.
引用
收藏
页数:7
相关论文
共 37 条
[1]   Almond protein as Pickering emulsion stabilizer: Impact of microgel fabrication method and pH on emulsion stability [J].
Almeida, Flavia Souza ;
da Silva, Aurenice Maria Mota ;
Mendes, Gabriel Augusto Campos ;
Sato, Ana Carla Kawazoe ;
Cunha, Rosiane Lopes .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 280
[2]   Fabrication of flavour oil high internal phase emulsions by casein/pectin hybrid particles: 3D printing performance [J].
Bi, An-Qi ;
Xu, Xian-Bing ;
Guo, Yu ;
Du, Ming ;
Yu, Cui-Ping ;
Wu, Chao .
FOOD CHEMISTRY, 2022, 371
[3]   Rennet coagulation of heated milk: A review [J].
Britten, Michel ;
Giroux, Helene J. .
INTERNATIONAL DAIRY JOURNAL, 2022, 124
[4]   Freeze-thaw stability of emulsions made with native and enzymatically modified egg yolk fractions [J].
Gmach, Oliver ;
Golda, Johannes ;
Kulozik, Ulrich .
FOOD HYDROCOLLOIDS, 2022, 123
[5]   Freeze-thaw stability of Pickering emulsion stabilized by modified soy protein particles and its application in plant-based ice cream [J].
Hei, Xue ;
Liu, Zhe ;
Li, Shanshan ;
Wu, Chao ;
Jiao, Bo ;
Hu, Hui ;
Ma, Xiaojie ;
Zhu, Jinjin ;
Adhikari, Benu ;
Wang, Qiang ;
Shi, Aimin .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2024, 257
[6]   Synergistic effect of residual sugar on freeze-thaw stability of high internal phase emulsions using glycosylated cod protein as interface stabilizer [J].
Hu, Sijie ;
Xiao, Feng ;
Du, Ming ;
Pan, Jinfeng ;
Song, Liang ;
Wu, Chao ;
Zhu, Beiwei ;
Xu, Xianbing .
FOOD CHEMISTRY, 2024, 432
[7]   The freeze-thaw stability of flavor high internal phase emulsion and its application to flavor preservation and 3D printing [J].
Hu, Sijie ;
Xiao, Feng ;
Du, Ming ;
Pan, Jinfeng ;
Song, Liang ;
Wu, Chao ;
Zhu, Beiwei ;
Xu, Xianbing .
FOOD CHEMISTRY-X, 2023, 19
[8]   Freeze-thaw-stable high internal phase emulsions stabilized by soy protein isolate and chitosan complexes at pH 3.0 as promising mayonnaise replacers [J].
Huang, Zhi-Xuan ;
Lin, Wei-Feng ;
Zhang, Yin ;
Tang, Chuan-He .
FOOD RESEARCH INTERNATIONAL, 2022, 156
[9]   New insights about flocculation process in sodium caseinate-stabilized emulsions [J].
Huck-Iriart, Cristian ;
Montes-de-Oca-Avalos, Juan ;
Lidia Herrera, Maria ;
Jorge Candal, Roberto ;
Pinto-de-Oliveira, Cristiano Luis ;
Linares-Torriani, Iris .
FOOD RESEARCH INTERNATIONAL, 2016, 89 :338-346
[10]   Preparation and stabilization of emulsions stabilized by mixed sodium caseinate and soy protein isolate [J].
Ji, Juan ;
Zhang, Jianpan ;
Chen, Jiashu ;
Wang, Yalong ;
Dong, Ni ;
Hu, Chunli ;
Chen, Hangping ;
Li, Ge ;
Pan, Xin ;
Wu, Chuanbin .
FOOD HYDROCOLLOIDS, 2015, 51 :156-165