Increasing the Number of Osteoblasts and Decreasing RANKL Expression on Diabetic Periodontitis in Rats Post-Administration of Nanoliposome Papaya Seed Extract

被引：0

作者：

Listari, Khusnul Munika ^{[1
]}

Pusporini, Ratih ^{[2
]}

Nugraeni, Yuli ^{[3
]}

Hidayat, Lukman Hakim ^{[4
]}

Festari, Vitha Dwi ^{[5
]}

Firdaus, Jasmine Dewinta ^{[5
]}

机构：

[1] Univ Brawijaya, Fac Dent, Dept Periodontol, Malang, Indonesia

[2] Univ Brawijaya, Fac Dent, Dept Oral Biol, Malang, Indonesia

[3] Univ Brawijaya, Fac Dent, Dept Conservat Dent, Malang, Indonesia

[4] Univ Brawijaya, Fac Dent, Dept Oral Med Dent, Malang, Indonesia

[5] Univ Brawijaya, Fac Dent, Malang, Indonesia

来源：

PESQUISA BRASILEIRA EM ODONTOPEDIATRIA E CLINICA INTEGRADA | 2025年 / 25卷

关键词：

Plant Extracts; Carica; Osteoblast; Diabetes Complications; Periodontics; DISEASE;

D O I：

10.1590/pboci.2025.017

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: To evaluate the impact of nanoliposome papaya seed extract on the number of osteoblasts and RANKL expression in a diabetic periodontitis rat model. Material and Methods: Thirty-six Sprague Dawley rats were randomly divided into three groups; each group was Group T-0 (rats induced to become diabetic periodontitis without treatment), Group T-1 (rats induced to become diabetic periodontitis and given 96% papaya seed extract) and Group T-2 (rats induced to become diabetic periodontitis and given 96% nanoliposome papaya seed extract). They were assessed at three points in time: day 3, day 7, and day 14. The extracts were given orally at 0.5 ml and drops as much as 0.03 ml on the gingival sulcus, and treatments were given once a day in each group at a predetermined time. Osteoblast cells were counted by hematoxylin-eosin (HE) staining, and RANKL expression was counted by immunohistochemistry (IHC) staining. The difference in osteoblast cells and RANKL expression between groups were analysed by one-way ANOVA and post hoc tests. Results: There was a significant difference in the number of osteoblasts on day 14 in the groups; a significant difference was also seen in RANKL expression on day 7 and day 14 in the groups. Conclusion: Administration of nanoliposome papaya seed extract affects the enhancement of the number of osteoblasts and reduction of RANKL expression in vivo.

引用

页数：10