A smartphone-assisted portable sensing hydrogel modules based on UCNPs and Co3O4 NPs for fluorescence quantitation of hypoxanthine in aquatic products

被引：7

作者：

Cao, Yunrui ^{[1
]}

Song, Yu ^{[1
]}

Fan, Xiaowei ^{[1
]}

Ma, Lei ^{[1
]}

Feng, Tingyu ^{[2
]}

Zeng, Junpeng ^{[1
]}

Xue, Changhu ^{[1
,3
]}

Xu, Jie ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, State Key Lab Marine Food Proc & Safety Control, 1299 Sansha Rd, Qingdao 266003, Shandong, Peoples R China

[2] Qingdao Inst Marine Bioresources Nutr & Hlth Innov, Qingdao, Peoples R China

[3] Qingdao Marine Sci & Technol Ctr, Qingdao 266235, Peoples R China

来源：

TALANTA | 2024年 / 276卷

关键词：

Sensor; Hypoxanthine; Nanoenzyme; Portability; Seafood; PEROXIDASE-LIKE ACTIVITY; SPECTROPHOTOMETRIC DETERMINATION; COLORIMETRIC DETECTION; NANOPARTICLES; XANTHINE; FISH;

D O I：

10.1016/j.talanta.2024.126259

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hypoxanthine is a promising index for evaluating the freshness of various aquatic products. Combined the hydrogels containing upconversion nanoparticles (UCNPs), Co3O4 NPs, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt/4-amino-antipyrine (TOPS/4-AAP) with a smartphone, a portable sensor was developed for the convenient, sensitive detection of hypoxanthine. With the H2O2 from xanthine oxidase (XOD)-catalyzed reactions of hypoxanthine, the fluorescence of UCNPs was effectively quenched by the purple product produced from the oxidization of TOPS/4-AAP catalyzed by Co3O4 NPs exhibiting peroxidase activity, among which the color change could be transformed into digital signals for quantification of hypoxanthine. The Green value in the RGB analysis of the fluorescence image was negatively proportional to hypoxanthine concentration in the range of 2.5-20 mg/L with a detection limit of 0.69 mg/L and a quantitation limit of 2.30 mg/L. Finally, this sensor was applied for hypoxanthine detection in real aquatic products, showing potential application for freshness evaluation of aquatic products.

引用

页数：8

共 46 条

[21] Simultaneous Spectrophotometric Determination of Xanthine, Hypoxanthine and Uric Acid in Real Matrix by Orthogonal Signal Correction-Partial Least Squares [J].