The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro-produced blastocysts

被引：0
作者：
Gonzalez-Plaza, A. [1 ,2 ]
Garcia-Canovas, M. [1 ,2 ]
Parrilla, I. [1 ,2 ]
Rodriguez-Martinez, H. [3 ]
Gil, M. A. [1 ,2 ]
Martinez, E. A. [1 ,2 ]
Cuello, C. [1 ,2 ]
机构：
[1] Univ Murcia, Fac Vet Med, Dept Med & Anim Surg, Int Excellence Campus Higher Educ & Res CMN, Murcia, Spain
[2] Inst Biomed Res Murcia IMIB Pascual Parrilla, Murcia, Spain
[3] Linkoping Univ, Fac Med & Hlth Sci, Dept Biomed & Clin Sci BKV, BKH Obstet & Gynecol, Linkoping, Sweden
来源：
REPRODUCTION IN DOMESTIC ANIMALS | 2024年 / 59卷
关键词：
Cryotop; in vitro-produced blastocysts; porcine; SOPS; vitrification;
D O I：
10.1111/rda.14600
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
This study aimed to compare the effectiveness, in terms of viability and quality post-warming, when vitrifying in vitro-produced (IVP) pig blastocysts with either a modified Cryotop (n = 161; 20 blastocysts/device) or the conventional Superfine Open Pulled Straw (SOPS; n=160; 5-6 blastocysts/device systems. Blastocyst viability, morphology, and apoptosis parameters were evaluated after 24 h of in vitro culture. The Cryotop system yields better results in terms of higher embryo viability and total cell numbers (p < .05) and lower apoptosis (p < .05) parameters than the SOPS procedure, defining a high effectiveness to simultaneously vitrify 20 pig IVP blastocysts at one time, thus increasing the yield of IVP blastocysts readily available for embryo transfer.
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页数：4
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