A high-resolution phase-contrast microscopy system for label-free imaging in living cells

被引：1

作者：

Shimasaki, Kentaro ^{[1
]}

Okemoto-Nakamura, Yuko ^{[1
]}

Saito, Kyoko ^{[1
]}

Fukasawa, Masayoshi ^{[1
]}

Katoh, Kaoru ^{[2
,3
]}

Hanada, Kentaro ^{[4
]}

机构：

[1] Natl Inst Infect Dis, Dept Biochem & Cell Biol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba Cent 6, 1-1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan

[3] Natl Inst Adv Ind Sci & Technol, AIRC, Koto Ku, Tokyo 1350064, Japan

[4] Natl Inst Infect Dis, Ctr Qual Management Syst, 1-23-1 Toyama, Shinjuku Ku, Tokyo 1628640, Japan

来源：

CELL STRUCTURE AND FUNCTION | 2024年 / 49卷 / 01期

关键词：

label-free imaging; organelle dynamics; virus infections; apodized phase contrast; JAPANESE-ENCEPHALITIS-VIRUS;

D O I：

10.1247/csf.24018

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cell biologists have long sought the ability to observe intracellular structures in living cells without labels. This study presents procedures to adjust a commercially available apodized phase-contrast (APC) microscopy system for better visualizing the dynamic behaviors of various subcellular organelles in living cells. By harnessing the versatility of this technique to capture sequential images, we could observe morphological changes in cellular geometry after virus infection in real time without probes or invasive staining. The tune-up APC microscopy system is a highly efficient platform for simultaneously observing the dynamic behaviors of diverse subcellular structures with exceptional resolution.

引用

页码：21 / 29

页数：9

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