Direct and Ultrasensitive Bioluminescent Detection of Intact Respiratory Viruses

被引：1

作者：

Grawe, Alexander ^{[1
,2
]}

van der Veer, Harm ^{[1
,2
]}

Jongkees, Seino A. K. ^{[3
]}

Flipse, Jacky ^{[4
,5
]}

Rossey, Iebe ^{[6
]}

de Vries, Robert P. ^{[7
]}

Saelens, Xavier ^{[6
]}

Merkx, Maarten ^{[1
,2
]}

机构：

[1] Eindhoven Univ Technol, Dept Biomed Engn, Lab Prot Engn, NL-5600MB Eindhoven, Netherlands

[2] Eindhoven Univ Technol, Inst Complex Mol Syst, NL-5600MB Eindhoven, Netherlands

[3] Vrije Univ Amsterdam, Amsterdam Inst Mol & Life Sci, Dept Chem & Pharmaceut Sci, NL-1081HZ Amsterdam, Netherlands

[4] Rijnstate Hosp, Lab Med Microbiol & Immunol, NL-6880AA Arnhem, Netherlands

[5] Dicoon, Lab Med Microbiol & Immunol, NL-6662PA Elst, Netherlands

[6] Univ Ghent, VIB Ctr Med Biotechnol, Dept Biochem & Microbiol, Inflammat Res Ctr, B-9052 Zwijnaarde, Belgium

[7] Utrecht Inst Pharmaceut Sci, Dept Chem Biol & Drug Discovery, NL-3584CG Utrecht, Netherlands

来源：

ACS SENSORS | 2024年 / 9卷 / 10期

关键词：

Respiratory virus; bioluminescence; virus diagnostics; protein engineering; protein ligation; biosensor; SYNCYTIAL VIRUS; INFLUENZA; PROTEIN; PERFORMANCE; ANTIBODIES; BINDING; ENTRY;

D O I：

10.1021/acssensors.4c01855

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Respiratory viruses such as SARS-CoV-2, influenza, and respiratory syncytial virus (RSV) represent pressing health risks. Rapid diagnostic tests for these viruses detect single antigens or nucleic acids, which do not necessarily correlate with the amount of the intact virus. Instead, specific detection of intact respiratory virus particles may be more effective at assessing the contagiousness of a patient. Here, we report GLOVID, a modular biosensor platform to detect intact virions against a background of "free" viral proteins in solution. Our approach harnesses the multivalent display of distinct proteins on the surface of a viral particle to template the reconstitution of a split luciferase, allowing specific, single-step detection of intact influenza A and RSV virions corresponding to 0.1-0.3 fM of genomic units. The protein ligation system used to assemble GLOVID sensors is compatible with a broad range of binding domains, including nanobodies, scFv fragments, and cyclic peptides, which allows straightforward adjustment of the sensor platform to target different viruses.

引用

页码：5550 / 5560

页数：11

共 48 条

[1] Analytical and clinical evaluation of a point-of-care molecular diagnostic system and its influenza A/B assay for rapid molecular detection of the influenza virus [J].