Effects of inorganic phosphate on stem cells isolated from human exfoliated deciduous teeth

被引：0

作者：

Suwittayarak, Ravipha ^{[1
]}

Nowwarote, Nunthawan ^{[2
,3
]}

Kornsuthisopon, Chatvadee ^{[1
,4
]}

Sukarawan, Waleerat ^{[1
,5
]}

Foster, Brian L. ^{[6
]}

Egusa, Hiroshi ^{[7
,8
]}

Osathanon, Thanaphum ^{[1
,2
,3
]}

机构：

[1] Chulalongkorn Univ, Fac Dent, Ctr Excellence Dent Stem Cell Biol, 34 Henri Dunant Rd, Bangkok 10330, Thailand

[2] Univ Paris Cite, Necker Hosp, Fac Dent, Dept Oral Biol, Paris, France

[3] Univ Paris Cite, Necker Hosp, Inst Imagine, Reference Ctr Skeletal Dysplasia,INSERM UMR1163, Paris, France

[4] Chulalongkorn Univ, Fac Dent, Dept Anat, Bangkok, Thailand

[5] Chulalongkorn Univ, Fac Dent, Dept Paediat Dent, Bangkok, Thailand

[6] Ohio State Univ, Coll Dent, Div Biosci, Columbus, OH USA

[7] Tohoku Univ, Grad Sch Dent, Div Mol & Regenerat Prosthodont, Sendai, Miyagi, Japan

[8] Tohoku Univ, Grad Sch Dent, Div Adv Prosthet Dent, Sendai, Miyagi, Japan

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Stem cells isolated from human exfoliated deciduous teeth; Inorganic phosphate; osteogenic differentiation; Adipogenic differentiation; GENE-EXPRESSION; OSTEOGENIC DIFFERENTIATION; ERK PATHWAYS; CALCIUM-IONS; APOPTOSIS; MINERALIZATION; OSTEOBLASTS; INHIBITION; CYCLE; ROLES;

D O I：

10.1038/s41598-024-75303-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Calcium phosphate-based materials (CaP) are introduced as potential dental pulp capping materials for deciduous teeth. The present study investigated the influence of inorganic phosphate (Pi) on regulating stem cells isolated from human exfoliated deciduous teeth (SHED). SHEDs were treated with Pi. Cell cycle progression and apoptosis were examined using flow cytometry analysis. Osteo/odontogenic and adipogenic differentiation were analyzed using alizarin red S and oil red O staining, respectively. The mRNA expression profile was investigated using a high-throughput RNA sequencing technique. Pi increased the late apoptotic cell population while cell cycle progression was not altered. Pi upregulated osteo/odontoblastic gene expression and enhanced calcium deposition. Pi-induced mineralization was reversed by pretreatment of cells with Foscarnet, or p38 inhibitor. Pi treatment inhibited adipogenic differentiation as determined by decreased PPAR gamma expression and reduced intracellular lipid accumulation. Bioinformatic analysis of gene expression profiles demonstrated several involved pathways, including PI3K/AKT, MAPK, EGFR, and VEGF signaling. In conclusion, Pi enhanced osteo/odontogenic but inhibited adipogenic differentiation in SHED.

引用

页数：12

共 76 条

[1] Short-term effects of calcium ions on the apoptosis and onset of mineralization of human dental pulp cells in vitro and in vivo [J].