Isolation, molecular detection, and sequence analysis of Avibacterium paragallinarum from suspected cases of infectious coryza infected chickens from different areas of Ethiopia, 2022-2024

被引：0

作者：

Deresse, Getaw ^{[1
]}

Assefa, Eyob ^{[1
]}

Tesfaw, Liyuwork ^{[1
]}

Dufera, Dawit ^{[1
]}

Adamu, Kassaye ^{[1
]}

Akalu, Mirtneh ^{[1
]}

Bayissa, Berecha ^{[1
]}

Abayneh, Takele ^{[1
]}

Zewdie, Girma ^{[1
]}

Abebe, Abiyot ^{[1
]}

Jemal, Mohamed ^{[1
]}

Megra, Abdo ^{[2
]}

Legesse, Abinet ^{[1
]}

Birhanu, Kenaw ^{[1
]}

Gelaye, Esayas ^{[3
]}

机构：

[1] Natl Vet Inst, POB 19, Bishoftu, Ethiopia

[2] Univ Gondar, Coll Vet Med & Anim Sci, Dept Pathobiol, Gondar, Ethiopia

[3] Food & Agr Org United Nations FAO, Emergency Ctr Transboundary Anim Dis ECTAD, Addis Ababa, Ethiopia

来源：

BMC MICROBIOLOGY | 2025年 / 25卷 / 01期

关键词：

<italic>Avibacterium paragallinarum</italic>; Infectious coryza; HPG-2; gene; PCR; Sequencing; Chicken; HAEMOPHILUS-PARAGALLINARUM; IDENTIFICATION; BACTERIA; PCR;

D O I：

10.1186/s12866-025-03862-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

BackgroundAvibacterium paragallinarum is a causative agent of infectious coryza (IC), a disease that affects the upper respiratory tracts and paranasal sinuses of chickens, resulting significant economic losses in the poultry industry. The objective of this study was to isolate and identify Av. paragallinarum using bacteriological and molecular methods between February 2022 and April 2024. A total of 74 swab samples were collected from chickens showing ocular and nasal discharges and swelling of the infraorbital sinuses.MethodClinical samples were collected from chickens showing symptoms of IC from six locations of Ethiopia for the isolation and identification of the causative agent. Swab samples from the nasal cavity and cheesy material from the infraorbital sinus were screened using conventional PCR and inoculated onto chocolate agar enriched with 5% sheep blood. Colonies suspected of being Av. paragallinarum were transferred to brain heart agar supplemented with horse serum. Gram staining was used to examine the morphology of bacteria in pure colonies grown on chocolate and brain heart infusion agar.ResultsThe isolation of Av. paragallinarum on chocolate and brain heart infusion agar resulted in the observation of small, translucent, dewdrop-shaped colonies after 24 h of incubation at 37 degrees C in a 5% CO2 incubator. A smear prepared from a single colony of revealed Gram-negative, short rod-shaped or coccobacilli Av. paragallinarum bacteria. Biochemical tests conducted on this isolate yielded negative results for catalase, oxidase, urease, indole, methyl red, and Voges-Proskauer tests. However, the bacterium exhibited positive fermentative activity with glucose, sucrose, and maltose. Biochemical assay revealed the presence of Av. Paragallinarum. The bacterial colonies confirmed a 511 bp PCR product. The partial HPG-2 gene nucleotide sequences of eleven isolates were sequenced and deposited in GenBank with the accession number PQ565862-72. A phylogenetic tree was constructed to determine the genetic relatedness of Ethiopian isolates with isolates from other African countries and elsewhere.ConclusionThe current investigation confirmed that the outbreaks were caused by Avibacterium paragallinarum and provided scientific evidence on the presence of different strains of Av. paragallinarum in Ethiopia. This is the first study in Ethiopia to detect and identify Av. paragallinarum from diseased chickens using molecular approaches. Further molecular characterization of locally circulating Av. paragallinarum isolates is recommended to be used as a vaccine strain for the prevention and control of infectious coryza.

引用

页数：12

共 38 条

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