Structure, interaction and nervous connectivity of beta cell primary cilia

被引：7

作者：

Mueller, Andreas ^{[1
,2
,3
,4
,5
]}

Klena, Nikolai ^{[6
]}

Pang, Song ^{[7
,12
]}

Garcia, Leticia Elizabeth Galicia ^{[1
,2
,3
,4
,5
,8
]}

Topcheva, Oleksandra ^{[1
,2
,3
,4
,5
]}

Aurrecoechea Duran, Solange ^{[1
,2
,3
,4
,5
]}

Sulaymankhil, Davud ^{[1
,2
,3
,4
,5
]}

Seliskar, Monika ^{[1
,2
,3
,4
,5
]}

Mziaut, Hassan ^{[1
,2
,3
,4
,5
]}

Schoeniger, Eyke ^{[1
,2
,3
,4
,5
]}

Friedland, Daniela ^{[1
,2
,3
,4
,5
]}

Kipke, Nicole ^{[1
,2
,3
,4
,5
]}

Kretschmar, Susanne ^{[9
]}

Muenster, Carla ^{[1
,2
,3
,4
,5
]}

Weitz, Juergen ^{[10
]}

Distler, Marius ^{[10
]}

Kurth, Thomas ^{[9
]}

Schmidt, Deborah ^{[11
]}

Hess, Harald F. ^{[7
]}

Xu, C. Shan ^{[7
,13
]}

Pigino, Gaia ^{[6
]}

Solimena, Michele ^{[1
,2
,3
,4
,5
,8
]}

机构：

[1] Tech Univ Dresden, Univ Hosp, Mol Diabetol, Dresden, Germany

[2] Tech Univ Dresden, Fac Med Carl Gustav Carus, Dresden, Germany

[3] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Paul Langerhans Inst Dresden PLID Helmholtz Munich, Dresden, Germany

[4] Tech Univ Dresden, Fac Med, Dresden, Germany

[5] German Ctr Diabet Res DZD eV, Neuherberg, Germany

[6] Human Technopole HT, Milan, Italy

[7] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[8] Tech Univ Dresden, DFG Cluster Excellence Phys Life, Dresden, Germany

[9] Tech Univ Dresden, Ctr Mol & Cellular Bioengn CMCB, Technol Platform, Core Facil Electron Microscopy & Histol, Dresden, Germany

[10] Tech Univ Dresden, Univ Hosp Carl Gustav Carus, Dept Visceral Thorac & Vasc Surg, Med Fac, Dresden, Germany

[11] Max Delbruck Ctr Mol Med Helmholtz Assoc MDC, Elect Microscopy, Berlin, Germany

[12] Yale Sch Med, New Haven, CT USA

[13] Yale Sch Med, Dept Cellular & Mol Physiol, New Haven, CT USA

来源：

NATURE COMMUNICATIONS | 2024年 / 15卷 / 01期

基金：

欧洲研究理事会;

关键词：

PANCREATIC-ISLETS; FINE-STRUCTURE; ULTRASTRUCTURE; EXPRESSION; LANGERHANS; ENDOCRINE; RECONSTRUCTION; ARCHITECTURE; PARACRINE; PROTEIN;

D O I：

10.1038/s41467-024-53348-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Primary cilia are sensory organelles present in many cell types, partaking in various signaling processes. Primary cilia of pancreatic beta cells play pivotal roles in paracrine signaling and their dysfunction is linked to diabetes. Yet, the structural basis for their functions is unclear. We present three-dimensional reconstructions of beta cell primary cilia by electron and expansion microscopy. These cilia are spatially confined within deep ciliary pockets or narrow spaces between cells, lack motility components and display an unstructured axoneme organization. Furthermore, we observe a plethora of beta cell cilia-cilia and cilia-cell interactions with other islet and non-islet cells. Most remarkably, we have identified and characterized axo-ciliary synapses between beta cell cilia and the cholinergic islet innervation. These findings highlight the beta cell cilia's role in islet connectivity, pointing at their function in integrating islet intrinsic and extrinsic signals and contribute to understanding their significance in health and diabetes. M & uuml;ller, Klena et al. provide comprehensive insights into beta cell cilia structure, their interaction with islet cells and islet innervation. Their data imply that these cilia are elective compartments for contact with the cholinergic nervous system.

引用

页数：18

共 101 条

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