Structural and molecular properties of mumps virus inclusion bodies

被引：1

作者：

Katoh, Hiroshi ^{[1
,2
]}

Kimura, Ryuichi ^{[3
,4
]}

Sekizuka, Tsuyoshi ^{[5
]}

Matsuoka, Kohei ^{[1
,2
]}

Hosogi, Mika ^{[1
,2
]}

Kitai, Yuki ^{[1
,2
]}

Akahori, Yukiko ^{[1
,2
]}

Kato, Fumihiro ^{[6
]}

Kataoka, Michiyo ^{[7
]}

Kobayashi, Hirotaka ^{[7
]}

Nagata, Noriyo ^{[7
]}

Suzuki, Tadaki ^{[7
]}

Ohkawa, Yasuyuki ^{[8
]}

Oki, Shinya ^{[3
,4
]}

Takeda, Makoto ^{[1
,2
]}

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Microbiol, Tokyo 1130033, Japan

[2] Univ Tokyo, Fac Med, Tokyo 1130033, Japan

[3] Kumamoto Univ, Inst Resource Dev & Anal, Kumamoto 8600811, Japan

[4] Kyoto Univ, Grad Sch Med, Dept Drug Discovery Med, Kyoto 6068507, Japan

[5] Natl Inst Infect Dis, Pathogen Genom Ctr, Tokyo 1628640, Japan

[6] Natl Inst Infect Dis, Dept Virol 3, Tokyo 1628640, Japan

[7] Natl Inst Infect Dis, Dept Pathol, Tokyo 1628640, Japan

[8] Kyushu Univ, Med Inst Bioregulat, Div Transcript, Fukuoka 8120054, Japan

来源：

SCIENCE ADVANCES | 2024年 / 10卷 / 49期

基金：

日本科学技术振兴机构;

关键词：

FUNCTIONAL-CHARACTERIZATION; RABIES VIRUS; PHASE; PHOSPHOPROTEIN; TRANSCRIPTION;

D O I：

10.1126/sciadv.adr0359

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family Paramyxoviridae, displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis of MuV IBs demonstrated that nucleocapsid and phospho (P)-proteins formed a cage-like structure and that the viral polymerase adopted a reticular pattern and colocalized with viral RNAs. In addition, we characterized host RNAs localized in MuV IBs by a spatial transcriptome analysis, and found that RNAs containing G-quadruplex motif sequences (G4-RNAs) were concentrated. An in vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in P droplets. Together, our data show that MuV generates IBs with a characteristic cage-like structure and host G4-RNAs play an important role in forming MuV IBs.

引用

页数：13

共 48 条

[1] Potential roles of G-quadruplex structures in RNA granules for physiological and pathological phase separation [J].