Genome-wide analysis of alternative splicing differences in hepatic ischemia reperfusion injury

被引:0
作者
Hua, Yongliang [1 ,2 ]
Li, Xinglong [1 ]
Yin, Bing [1 ]
Lu, Shounan [1 ]
Qian, Baolin [1 ]
Zhou, Yongzhi [1 ]
Li, Zhongyu [1 ]
Meng, Zhanzhi [1 ]
Ma, Yong [1 ]
机构
[1] Harbin Med Univ, Dept Minimally Invas Hepat Surg, Key Lab Hepatosplen Surg, Minist Educ,Affiliated Hosp 1, Harbin, Heilongjiang, Peoples R China
[2] Harbin Med Univ, Dept Pediat Surg, Key Lab Hepatosplen Surg, Minist Educ,Affiliated Hosp 6, Harbin, Heilongjiang, Peoples R China
来源
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
基金
黑龙江省自然科学基金;
关键词
D O I
10.1038/s41598-024-82846-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Alternative splicing (AS) contributes to transcript and protein diversity, affecting their structure and function. However, the specific transcriptional regulatory mechanisms underlying AS in the context of hepatic ischemia reperfusion (IR) injury in mice have not been extensively characterized. In this study, we investigated differentially alternatively spliced (DAS) genes and differentially expressed transcripts (DETs) in a mouse model of hepatic IR injury using the high throughput RNA sequencing (RNA-seq) analysis and replicate multivariate analysis of transcript splicing (rMATS) analysis. We further conducted Gene ontology (GO) term enrichment, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the protein-protein interaction (PPI) network. A total of 898 DAS genes (p <= 0.05) were screened out in the hepatic IR group compared to the sham group, while functional enrichment analysis revealed that DETs and DAS genes were significantly associated with the ATP-dependent chromain, splicesome and metabolic pathways. The expression level of the DAS genes: Gabpb2, Smg1, Tnrc6c, Mettl17, Smpd4, Kcnt2, D16Ertd472e, Rab3gap2, Echdc2 and Ssx2ip were verified by RT-PCR and qRT-PCR. Our findings provide a comprehensive genome-wide view of AS events in hepatic IR injury in mice, enhancing our understanding of AS dynamics and the molecular mechanisms governing alternative pre-mRNA splicing.
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页数:10
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