Molecular mechanisms of plant productivity enhancement by nano fertilizers for sustainable agriculture

Essential plant nutrients encapsulated or combined with nano-dimensional adsorbents define nano fertilizers (NFs). Nanoformulation of non-essential elements enhancing plant growth and stress tolerance also comes under the umbrella of NFs. NFs have an edge over conventional chemical fertilizers, viz., higher plant biomass and yield using much lesser fertilization, thereby reducing environmental pollution. Foliar and root applications of NFs lead to their successful uptake by the plant, depending on the size, surface charge, and other physicochemical properties of NFs. Smaller NFs can pass through channels on the waxy cuticle depending on the hydrophobicity, while larger NFs pass through the stomatal conduits of leaves. Charge-based adsorption, followed by apoplastic movement and endocytosis, translocates NFs through the root, while the size of NFs influences passage into vascular tissues. Recent transcriptomic, proteomic, and metabolomic studies throw light on the molecular mechanisms of growth promotion by NFs. The expression levels of nutrient transporter genes are regulated by NFs, controlling uptake and minimizing excess nutrient toxicity. Accelerated growth by NFs is brought about by their extensive regulation of cell division, photosynthesis, carbohydrate, and nitrogen metabolism, as well as the phytohormone-dependent signaling pathways related to development, stress response, and plant defense. NFs mimic Ca,2+ eliciting second messengers and associated proteins in signaling cascades, reaching transcription factors and finally orchestrating gene expression to enhance growth and stress tolerance. Developing advanced nano fertilizers of the future must involve exploring molecular interactions with plants to reduce toxicity and improve effectiveness. Nano fertilizers enhance plant growth and stress tolerance by regulating cell division, morphogenesis, photosynthesis, nitrogen assimilation, vacuolar sequestration of excess nutrients, phytohormone and calcium signaling, and secondary metabolite biosynthesis.