ObjectiveThe present study examined the validity and reliability of the Nurses' Professional Values Scale (Nurses' PVS) by adapting it to Turkish. MethodsThis methodological study was conducted with 297 nurses who agreed to participate in the study by using the Snowball Sampling Method. The study data were collected using the Descriptive Data Form and Nurses' PVS. The data were evaluated using the Content Validity Index, Confirmatory Factor Analysis, Cronbach's Alpha, McDonald's Omega Reliability, and test-retest analysis. ResultsFactor load values of the scale items were found to vary between 0.590 and 0.960. The fit index values were found to be X-2 = 102.87, df = 398 (p < 0.05), X-2/df = 2.77, RMSEA = 0.077, CFI = 0.99, RMR = 0.045, SRMR = 0.051 and TLI = 0.97. It was also found that the Cronbach's Alpha Coefficients of the sub-dimensions of the scale varied between 0.896 and 0.977, and the total Cronbach's Alpha Coefficient was 0.958. The total McDonald's Omega Coefficient was .958, and the McDonald's Omega Coefficients of the sub-dimensions varied between .896-.977. The Turkish form of the 30-item and 4-subdimensional scale was confirmed without any changes in the original scale form. ConclusionThe Turkish version of the Nurses' PVS is a valid and reliable tool for measuring the values that nurses care about. Clinical trial numberNot applicable.