Triangle correlations of lung microbiome, host physiology and gut microbiome in a rat model of idiopathic pulmonary fibrosis

被引：0

作者：

Hou, Sihan ^{[1
,2
,3
]}

Wang, Xueer ^{[1
,2
]}

Guo, Jiarui ^{[1
,2
]}

Han, Yue ^{[1
,2
]}

You, Jia ^{[4
]}

Tian, Zhigang ^{[5
]}

Zheng, Xiwei ^{[5
]}

Zheng, Siriguleng ^{[6
]}

Ling, Yaqing ^{[1
,2
]}

Pei, Lingpeng ^{[1
,2
]}

Wu, Enqi ^{[1
,2
]}

机构：

[1] Minzu Univ China, Sch Pharm, Beijing, Peoples R China

[2] Minzu Univ China, Minist Educ, Key Lab Ethnomed, 27 Zhongguancun South Ave, Beijing 100081, Peoples R China

[3] Beihang Univ, Inst Environm Biol & Life Support Technol, Sch Biol Sci & Med Engn, Beijing 100083, Peoples R China

[4] Seventh Med Ctr PLA Gen Hosp, Biotherapy Ctr, Beijing 100081, Peoples R China

[5] Ningxia Med Univ, Dept Resp & Crit Care Med, Gen Hosp, 804 Shenglijie, Yinchuan 750004, Peoples R China

[6] Polytech Coll, Dept Informat Technol, Beijing, Peoples R China

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Idiopathic pulmonary fibrosis; Lung microbiome; Gut microbiome; Lung-gut axis; Mediation analysis; PROGRESSION;

D O I：

10.1038/s41598-024-80023-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Changes in lung and gut microbial communities have been associated with idiopathic pulmonary fibrosis (IPF). This study aimed to investigate correlations between microbial changes in the lung and gut and host physiological indices in an IPF model, exploring potential mechanisms of the lung-gut axis in IPF pathogenesis. IPF model rats were established via trans-tracheal injection of bleomycin, with assessments of hematological indices, serum cytokines, lung histopathology, and microbiome alterations. Significant differences in microbial structure and composition were observed in the IPF model compared to controls, with 14 lung and 7 gut microbial genera showing significant abundance changes. Further analysis revealed 20 significant correlations between pulmonary and gut genera. Notably, 11 pairs of correlated genera were linked to the same IPF-related physiological indices, such as hydroxyproline, mean corpuscular volume (MCV), and red cell distribution width-standard deviation (RDW-SD). We identified 24 instances where a lung and a gut genus were each associated with the same physiological index, forming "lung genus-index-gut genus" relationships. Mediation analysis showed that indices like hydroxyproline, MCV, and RDW-SD mediated correlations between 10 lung genera (e.g., Cetobacterium, Clostridium XVIII ) and the gut genus Allobaculum. This study first describes gut-lung microbial interactions in pulmonary fibrosis. Mediation analysis suggests pathways underlying "lung genus-host index-gut genus" and "gut genus-host index-lung genus" correlations, thus providing clues to further elucidate the mechanisms of the "gut-lung axis" in IPF pathogenesis.

引用

页数：13

共 48 条

[1] Sergio H(2020)Epidemiology of idiopathic pulmonary fibrosis: a population-based study in primary care Intern. Emerg. Med. 15 437-445
[2] Biondini D(2020)Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF): an overview of current and future therapeutic strategies Expert Rev Respir Med 14 405-414
[3] Carrington R(2018)Use of animal models in IPF research Pulm Pharmacol Ther 51 73-78
[4] Han MK(2014)Lung microbiome and disease progression in idiopathic pulmonary fibrosis: an analysis of the COMET study Lancet Respir Med 2 548-556
[5] Molyneaux PL(2014)The role of bacteria in the pathogenesis and progression of idiopathic pulmonary fibrosis Am J Respir Crit Care Med 190 906-913
[6] O’Dwyer DN(2019)Lung Microbiota Contribute to Pulmonary Inflammation and Disease Progression in Pulmonary Fibrosis Am J Respir Crit Care Med 199 1127-1138
[7] Molyneaux PL(2017)Host-Microbial Interactions in Idiopathic Pulmonary Fibrosis Am J Respir Crit Care Med 195 1640-1650
[8] Wu Y(2022)Gut microbiome and metabolites: The potential key roles in pulmonary fibrosis Front Microbiol 13 1200937-963
[9] Shi H(2023)The associations between gut microbiota and chronic respiratory diseases: a Mendelian randomization study Front Microbiol 14 5-57
[10] Quan Y(2022)The gut-lung axis: Gut microbiota changes associated with pulmonary fibrosis in mouse models induced by bleomycin Front Pharmacol 13 1401-1500

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