Polidocanol inhibits Enterococcus faecalis virulence factors by targeting fsr quorum sensing system

被引:1
作者
Ashraf, Dina [1 ]
Shaaban, Mona I. [1 ]
Hassan, Ramadan [1 ]
El-Aziz, Abeer M. Abd [1 ]
机构
[1] Mansoura Univ, Fac Pharm, Dept Microbiol & Immunol, Mansoura 35516, Egypt
来源
BMC MICROBIOLOGY | 2024年 / 24卷 / 01期
关键词
Enterococcus faecalis; Quorum sensing; Gelatinase; Virulence; BIOSYNTHESIS-ACTIVATING PHEROMONE; BIOFILM FORMATION; GENE-EXPRESSION; GELATINASE; PROTEASE; BACTERIA; RELEASE; GELE; PCR;
D O I
10.1186/s12866-024-03548-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background The wide spread of antimicrobial resistance in Enterococcus faecalis is a critical global concern, leading to increasingly limited treatment options. The fsr quorum sensing (QS) plays a critical role in the pathogenicity of E. faecalis, allowing bacteria to coordinate gene expression and regulate many virulence factors. Therefore, fsr QS of E. faecalis represents a potential therapeutic target that provides an effective strategy to treat antibiotic-resistant infections induced by E. faecalis. Methods In this study, distribution of different virulence factors including, gelatinase, protease, cell surface hydrophobicity and biofilm formation in sixty clinical isolates of Enterococcus faecalis was investigated. Sixty-six compounds were tested for their activity against fsr QS. The minimal inhibitory concentration of the tested compounds was evaluated using the microbroth dilution method. The effect of sub-inhibitory concentrations of the tested compounds on fsr QS was investigated using the gelatinase assay method. Additionally, the effect of potential QS inhibitor on the virulence factors was estimated. Quantitative real-time PCR was used to investigate the effect of the potential inhibitor on fsr QS related genes (fsrB-fsrC) and (gelE-sprE) and virulence associated genes including, asa1 and epbA. Results The assessment of polidocanol activity against the fsr QS system was demonstrated by studying its effect on gelatinase production in E. faecalis clinical isolates. Sub-lethal concentrations of polidocanol showed a significant reduction in gelatinase and protease production by 54% to 70% and 64% to 85%, respectively. Additionally, it significantly reduced biofilm formation (P < 0.01) and interrupted mature biofilm at concentrations of <1/2>, 1 x and 2 x MIC. Furthermore, polidocanol significantly decreased cell surface hydrophobicity (P < 0.01). Polidocanol at <1/2> MIC showed a significant reduction in the expression of QS genes including fsrB, fsrC, gelE and sprE by 57% to 97% without affecting bacterial viability. Moreover, it reduced the expression of virulence associated genes (asa1 and epbA) (P < 0.01). Conclusion Polidocanol appears to be a promising option for treating of E. faecalis infections by targeting the fsr QS system and exhibiting anti-biofilm activity.
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相关论文
共 67 条
[1]   Molecular characterization of Salmonella spp. and Listeria monocytogenes strains from biofilms in cattle and poultry slaughterhouses located in the federal District and State of Goias, Brazil [J].
Agostinho Davanzo, Emilia Fernanda ;
dos Santos, Rebecca Lavarini ;
de Lemos Castro, Virgilio Hipolito ;
Palma, Joana Marchesini ;
Pribul, Bruno Rocha ;
Lima Dallago, Bruno Stefano ;
Fuga, Bruna ;
Medeiros, Margareti ;
Titze de Almeida, Simoneide Souza ;
Boaventura da Costa, Hayanna Maria ;
Rodrigues, Dalia Dos Prazeres ;
Lincopan, Nilton ;
Perecmanis, Simone ;
Santana, Angela Patricia .
PLOS ONE, 2021, 16 (11)
[2]  
Akshaya BS., 2023, Cinnamaldehyde inhibits Enterococcus faecalis biofilm formation and promotes clearance of its colonization by modulation of phagocytes in vitro, V181
[3]   Fsr quorum sensing system modulates the temporal development of Enterococcus faecalis biofilm matrix [J].
Ali, Islam A. A. ;
Levesque, Celine M. ;
Neelakantan, Prasanna .
MOLECULAR ORAL MICROBIOLOGY, 2022, 37 (01) :22-30
[4]   Trans-Cinnamaldehyde Attenuates Enterococcus faecalis Virulence and Inhibits Biofilm Formation [J].
Ali, Islam A. A. ;
Matinlinna, Jukka P. ;
Levesque, Celine M. ;
Neelakantan, Prasanna .
ANTIBIOTICS-BASEL, 2021, 10 (06)
[5]   Molecular Mechanism of Quorum-Sensing in Enterococcus faecalis: Its Role in Virulence and Therapeutic Approaches [J].
Ali, Liaqat ;
Goraya, Mohsan Ullah ;
Arafat, Yasir ;
Ajmal, Muhammad ;
Chen, Ji-Long ;
Yu, Daojin .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (05)
[6]   Enterococcus faecalis from Food, Clinical Specimens, and Oral Sites: Prevalence of Virulence Factors in Association with Biofilm Formation [J].
Anderson, Annette C. ;
Jonas, Daniel ;
Huber, Ingrid ;
Karygianni, Lamprini ;
Woelber, Johan ;
Hellwig, Elmar ;
Arweiler, Nicole ;
Vach, Kirstin ;
Wittmer, Annette ;
Al-Ahmad, Ali .
FRONTIERS IN MICROBIOLOGY, 2016, 6
[7]   Prevalence of Virulence Factors and Drug Resistance in Clinical Isolates of Enterococci: A Study from North India [J].
Banerjee, Tuhina ;
Anupurba, Shampa .
JOURNAL OF PATHOGENS, 2015, 2015
[8]  
CLSI, 2018, CLSI supplement M07, V11th, P19087
[9]   Minimally invasive treatment of aneurysmal bone cysts: Systematic literature review [J].
Cottalorda, Jerome ;
Sabah, Djamel Louahem ;
Monrigal, Pauline Joly ;
Jeandel, Clement ;
Delpont, Marion .
ORTHOPAEDICS & TRAUMATOLOGY-SURGERY & RESEARCH, 2022, 108 (04)
[10]  
Dashti AA, 2009, KUWAIT MED J, V41, P117