Establishment of callus induction and plantlet regeneration systems of Peucedanum Praeruptorum dunn based on the tissue culture method

被引:3
作者
Pan, Haoyu [1 ,2 ]
Liao, Ranran [1 ,3 ]
Zhang, Yingyu [4 ]
Arif, Muhammad [5 ]
Zhang, Yuxin [1 ]
Zhang, Shuai [1 ]
Wang, Yuanyuan [1 ,3 ]
Zhao, Pengcheng [1 ]
Wang, Zaigui [2 ]
Han, Bangxing [1 ,2 ,3 ]
Song, Cheng [1 ,2 ]
机构
[1] West Anhui Univ, Anhui Dabieshan Acad Tradit Chinese Med, Coll Biol & Pharmaceut Engn, Anhui Engn Res Ctr Ecoagr Tradit Chinese Med, Luan 237012, Peoples R China
[2] Anhui Agr Univ, Sch Life Sci, Hefei 230036, Peoples R China
[3] Anhui Univ Chinese Med, Sch Pharm, Hefei 230012, Peoples R China
[4] Univ Sci & Technol, Affiliated Hosp 1, Coll Clin Med Henan, Luoyang 471003, Peoples R China
[5] Sakarya Univ Appl Sci, Fac Agr, Dept Plant Protect, TR-54580 Sakarya, Turkiye
基金
国家重点研发计划;
关键词
<italic>Peucedanum Praeruptorum</italic> Dunn; Somatic embryo; Organogenesis; Plant growth regulator; Tissue culture; Embryogenic callus; SOMATIC EMBRYOGENESIS; SHOOT ORGANOGENESIS; EXPRESSION; EMBRYOS; CELLS; LEAF;
D O I
10.1186/s13007-024-01300-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
BackgroundPeucedanum praeruptorum Dunn has typical stacked umbels and medicinal value; however, the lack of an effective tissue culture system for P. praeruptorum has limited the large-scale propagation of its seedlings.ResultsWe systematically established an in vitro regeneration system for P. praeruptorum using young leaves and stems as explants. Tissue culture plantlets were successfully obtained within 123 and 90 d of somatic embryogenesis and organogenesis, respectively. Combined plant growth regulators (PGRs) were optimized to promote efficient plant regeneration at each stage of the culture process. Specifically, embryogenic callus induction was superior in Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 6-benzyladenine (BA) and 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). For somatic embryonic development, the highest differentiation rates were achieved using BA, 2,4-D, and 6-furfuryl aminopurine (6-KT). Induction of organogenesis resulted in the highest differentiation rates and proliferation coefficients of buds in MS medium supplemented with BA and alpha-naphthaleneacetic acid (NAA). Moreover, regeneration of P. praeruptorum seedlings was achieved by adjusting the BA and indole-3-butyric acid (IBA) concentrations in 1/2 MS medium.ConclusionOur results provide a technical system for the rapid propagation of P. praeruptorum, which can facilitate germplasm improvement, resource conservation, and further genetic transformation of Peucedanum species.
引用
收藏
页数:16
相关论文
共 60 条
[1]   Morphology and ontogeny of directly differentiating shoot buds and somatic embryos in Santalum album L. [J].
Akhtar, Rakhshanda ;
Shahzad, Anwar .
JOURNAL OF FORESTRY RESEARCH, 2019, 30 (04) :1179-1189
[2]  
Aydoan SK., 2019, Avrupa Bilim ve Teknoloji Dergisi, P367
[3]   Cryopreservation of embryogenic tissues from mature holm oak trees [J].
Barra-Jimenez, Azahara ;
Aronen, Tuija S. ;
Alegre, Jesus ;
Toribio, Mariano .
CRYOBIOLOGY, 2015, 70 (03) :217-225
[4]  
Bengi E, 2005, Pak J Biol Sci, V8, P91
[5]  
Cabuk B, 2016, Infection, V2, P188
[6]   High-frequency somatic embryogenesis from germinated zygotic embryos of Schisandra chinensis and evaluation of the effects of medium strength, sucrose, GA3, and BA on somatic embryo development [J].
Chen, Ai Hua ;
Yang, Jing Li ;
Da Niu, Yu ;
Yang, Chuan Ping ;
Liu, Gui Feng ;
Yu, Chang Yeon ;
Li, Cheng Hao .
PLANT CELL TISSUE AND ORGAN CULTURE, 2010, 102 (03) :357-364
[7]   Cytokinin action in response to abiotic and biotic stresses in plants [J].
Cortleven, Anne ;
Leuendorf, Jan Erik ;
Frank, Manuel ;
Pezzetta, Daniela ;
Bolt, Sylvia ;
Schmuelling, Thomas .
PLANT CELL AND ENVIRONMENT, 2019, 42 (03) :998-1018
[8]   Hormonal control of the plant cell cycle [J].
del Pozo, JC ;
Lopez-Matas, MA ;
Ramirez-Parra, E ;
Gutierrez, C .
PHYSIOLOGIA PLANTARUM, 2005, 123 (02) :173-183
[9]   The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera) [J].
Deng, Xianbao ;
Xiong, Yaqian ;
Li, Jing ;
Yang, Dong ;
Liu, Juan ;
Sun, Heng ;
Song, Heyun ;
Wang, Yunmeng ;
Ma, Junyu ;
Liu, Yanling ;
Yang, Mei .
PLANTS-BASEL, 2020, 9 (11) :1-13
[10]  
Emek Y, 2018, European Journal of Science and Technology, P337