Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill - Bucorvus leadbeateri

被引:0
|
作者
Patel, Jasmin [1 ]
Botes, Angela [1 ]
Mollett, Jean [1 ]
De Maayer, Pieter [1 ]
机构
[1] Univ Witwatersrand, Sch Mol & Cell Biol, ZA-2017 Johannesburg, South Africa
基金
新加坡国家研究基金会;
关键词
TOOL; DATABASE; GENES; KEGG;
D O I
10.1038/s41597-025-04412-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Southern Ground Hornbill (SGH - Bucorvus leadbeateri) is one of the largest hornbill species worldwide, known for its complex social structures and breeding behaviours. This bird has been of great interest due to its declining population and disappearance from historic ranges in southern Africa. Despite being the focus of numerous conservation efforts, with research forming an integral part of these initiatives, there is still a substantial lack of knowledge regarding the molecular biology aspects of this bird species. In this study, whole genome sequencing of the SGH was achieved using Illumina short-read (NovaSeq 6000) and Pacific Biosciences long-read technologies. A hybrid de novo genome assembly followed by reference-based refinement produced a 1.16 Gb high-quality draft genome assembly of the SGH comprised of 1,672 contigs (N50 value of 40.45 Mb). The availability of this genome will aid in improving our current understanding of this bird at a genomic level and provide a pivotal foundation for comparative genomic analysis with other hornbill species.
引用
收藏
页数:7
相关论文
共 30 条
  • [21] Whole-genome assembly and annotation of northern wild rice, Zizania palustris L., supports a whole-genome duplication in the Zizania genus
    Haas, Matthew
    Kono, Thomas
    Macchietto, Marissa
    Millas, Reneth
    McGilp, Lillian
    Shao, Mingqin
    Duquette, Jacques
    Hirsch, Candice N.
    Kimball, Jennifer
    Qiu, Yinjie
    PLANT JOURNAL, 2021, 107 (06) : 1802 - 1818
  • [22] Whole genome sequencing and de novo assembly of three virulent Indian isolates of Leptospira
    Lata, Kumari Snehkant
    Vaghasia, Vibhisha
    Bhairappanavar, Shivarudrappa B.
    Kumar, Swapnil
    Ayachit, Garima
    Patel, Saumya
    Das, Jayashankar
    INFECTION GENETICS AND EVOLUTION, 2020, 85
  • [23] Whole genome sequencing and annotation of Aspergillus flavus JAM-JKB-B HA-GG20
    Bharose, Achyut Ashokrao
    Hajare, Sunil Tulshiram
    Narayanrao, Dhawale Ramesh
    Gajera, H. G.
    Prajapati, Hrushna Kany
    Singh, Suresh Chandra
    Upadhye, Vijay
    SCIENTIFIC REPORTS, 2024, 14 (01)
  • [24] Chromosome-level genome assembly and annotation of the Yunling cattle with PacBio and Hi-C sequencing data
    Wei, Zaichao
    Zhang, Lilian
    Gao, Lutao
    Chen, Jian
    Peng, Lin
    Yang, Linnan
    SCIENTIFIC DATA, 2024, 11 (01)
  • [25] Whole-genome assembly and annotation for the little yellow croaker (Larimichthys polyactis) provide insights into the evolution of hermaphroditism and gonochorism
    Xie, Qing-Ping
    Zhan, Wei
    Shi, Jian-Zhi
    Liu, Feng
    Niu, Bao-Long
    He, Xue
    Liu, Meng
    Wang, Jing
    Liang, Qi-Qi
    Xie, Yue
    Xu, Peng
    Wang, Xu
    Lou, Bao
    MOLECULAR ECOLOGY RESOURCES, 2023, 23 (03) : 632 - 658
  • [26] Improved hybrid de novo genome assembly and annotation of African wild rice, Oryza longistaminata, from Illumina and PacBio sequencing reads
    Li, Wei
    Li, Kui
    Zhang, Qun-jie
    Zhu, Ting
    Zhang, Yun
    Shi, Cong
    Liu, Yun-long
    Xia, En-hua
    Jiang, Jian-jun
    Shi, Chao
    Zhang, Li-ping
    Huang, Hui
    Tong, Yan
    Liu, Yuan
    Zhang, Dan
    Zhao, Yuan
    Jiang, Wen-kai
    Zhao, You-jie
    Mao, Shu-yan
    Jiao, Jun-ying
    Xu, Ping-zhen
    Yang, Li-li
    Yin, Guo-ying
    Gao, Li-zhi
    PLANT GENOME, 2020, 13 (01)
  • [27] GENOMIC ANALYSIS MADE EASY (GAME V1): AN AUTOMATED SOFTWARE FOR PLANT GENOME ASSEMBLY AND ANNOTATION FROM ILLUMINA SEQUENCING
    Ali, Mohammad ajmal
    Mahato, Rajesh
    Lee, Joongku
    BANGLADESH JOURNAL OF PLANT TAXONOMY, 2024, 31 (02) : 225 - 238
  • [28] Chromosome-scale assembly and whole-genome sequencing of 266 giant panda roundworms provide insights into their evolution, adaptation and potential drug targets
    Han, Lei
    Lan, Tianming
    Li, Desheng
    Li, Haimeng
    Deng, Linhua
    Peng, Zhiwei
    He, Shaowen
    Zhou, Yanqiang
    Han, Ruobing
    Li, Lingling
    Lu, Yaxian
    Lu, Haorong
    Wang, Qing
    Yang, Shangchen
    Zhu, Yixin
    Huang, Yunting
    Cheng, Xiaofang
    Yu, Jieyao
    Wang, Yulong
    Sun, Heting
    Chai, Hongliang
    Yang, Huanming
    Xu, Xun
    Lisby, Michael
    Liu, Quan
    Kristiansen, Karsten
    Liu, Huan
    Hou, Zhijun
    MOLECULAR ECOLOGY RESOURCES, 2022, 22 (02) : 768 - 785
  • [29] De novo genome assembly and annotation of Australia's largest freshwater fish, the Murray cod (Maccullochella peelii) from Illumina and Nanopore sequencing reads
    Austin, Christopher M.
    Tan, Mun Hua
    Harrisson, Katherine A.
    Lee, Yin Peng
    Croft, Laurence J.
    Sunnucks, Paul
    Pavlova, Alexandra
    Gan, Han Ming
    GIGASCIENCE, 2017, 6 (08):
  • [30] De-novo whole genome assembly of the orange jewelweed, Impatiens capensis Meerb. (Balsaminaceae) using nanopore long-read sequencing
    Gadagkar, Sudhindra R.
    Baeza, J. Antonio
    Buss, Kristina
    Johnson, Nate
    PEERJ, 2023, 11