Whole genome sequencing, assembly and annotation of the Southern Ground Hornbill - Bucorvus leadbeateri

被引:0
|
作者
Patel, Jasmin [1 ]
Botes, Angela [1 ]
Mollett, Jean [1 ]
De Maayer, Pieter [1 ]
机构
[1] Univ Witwatersrand, Sch Mol & Cell Biol, ZA-2017 Johannesburg, South Africa
基金
新加坡国家研究基金会;
关键词
TOOL; DATABASE; GENES; KEGG;
D O I
10.1038/s41597-025-04412-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Southern Ground Hornbill (SGH - Bucorvus leadbeateri) is one of the largest hornbill species worldwide, known for its complex social structures and breeding behaviours. This bird has been of great interest due to its declining population and disappearance from historic ranges in southern Africa. Despite being the focus of numerous conservation efforts, with research forming an integral part of these initiatives, there is still a substantial lack of knowledge regarding the molecular biology aspects of this bird species. In this study, whole genome sequencing of the SGH was achieved using Illumina short-read (NovaSeq 6000) and Pacific Biosciences long-read technologies. A hybrid de novo genome assembly followed by reference-based refinement produced a 1.16 Gb high-quality draft genome assembly of the SGH comprised of 1,672 contigs (N50 value of 40.45 Mb). The availability of this genome will aid in improving our current understanding of this bird at a genomic level and provide a pivotal foundation for comparative genomic analysis with other hornbill species.
引用
收藏
页数:7
相关论文
共 30 条
  • [1] A field guide to whole-genome sequencing, assembly and annotation
    Ekblom, Robert
    Wolf, Jochen B. W.
    EVOLUTIONARY APPLICATIONS, 2014, 7 (09): : 1026 - 1042
  • [2] ChopStitch: exon annotation and splice graph construction using transcriptome assembly and whole genome sequencing data
    Khan, Hamza
    Mohamadi, Hamid
    Vandervalk, Benjamin P.
    Warren, Rene L.
    Chu, Justin
    Birol, Inanc
    BIOINFORMATICS, 2018, 34 (10) : 1697 - 1704
  • [3] A De Novo Whole Genome Assembly and Annotation of Parelaphostrongylus tenuis
    Garwood, Tyler J.
    Richards, Jessie E.
    Macchietto, Marissa G.
    Gerhold, Richard W.
    Kania, Stephen A.
    Garbe, John R.
    Fountain-Jones, Nicholas M.
    Larsen, Peter A.
    Wolf, Tiffany M.
    JOURNAL OF NEMATOLOGY, 2024, 56 (01)
  • [4] Assembly and annotation of whole-genome sequence of Fusarium equiseti
    Li, Xueping
    Xu, Shiyang
    Zhang, Jungao
    Li, Minquan
    GENOMICS, 2021, 113 (04) : 2870 - 2876
  • [5] Whole genome sequencing and genome annotation of the wild edible mushroom, Russula griseocarnosa
    Yu, Fei
    Song, Jie
    Liang, Junfeng
    Wang, Shengkun
    Lu, Junkun
    GENOMICS, 2020, 112 (01) : 603 - 614
  • [6] The Genome Assembly and Annotation of the Southern Elephant Seal Mirounga leonina
    Kim, Bo-Mi
    Lee, Yoon Jin
    Kim, Jeong-Hoon
    Kim, Jin-Hyoung
    Kang, Seunghyun
    Jo, Euna
    Lee, Seung Jae
    Lee, Jun Hyuck
    Chi, Young Min
    Park, Hyun
    GENES, 2020, 11 (02)
  • [7] Sequencing and functional annotation of the whole genome of the filamentous fungus Aspergillus westerdijkiae
    Han, Xiaolong
    Chakrabortti, Alolika
    Zhu, Jindong
    Liang, Zhao-Xun
    Li, Jinming
    BMC GENOMICS, 2016, 17
  • [8] De novo sequencing, assembly and functional annotation of Armillaria borealis genome
    Akulova, Vasilina S.
    Sharov, Vadim V.
    Aksyonova, Anastasiya I.
    Putintseva, Yuliya A.
    Oreshkova, Natalya V.
    Feranchuk, Sergey I.
    Kuzmin, Dmitry A.
    Pavlov, Igor N.
    Litovka, Yulia A.
    Krutovsky, Konstantin V.
    BMC GENOMICS, 2020, 21 (Suppl 7)
  • [9] Whole genome sequencing and annotation of Scleroderma yunnanense, the only edible Scleroderma species
    Zhang, Shanshan
    Yang, Wenzhong
    Chen, Jian
    Zhang, Chuanguang
    Zhang, Siqi
    Gao, Lanjing
    GENOMICS, 2023, 115 (06)
  • [10] De novo genome assembly and annotation of Holothuria scabra (Jaeger, 1833) from nanopore sequencing reads
    Luo, Honglin
    Huang, Guanghua
    Li, Jianbin
    Yang, Qiong
    Zhu, Jiajie
    Zhang, Bin
    Feng, Pengfei
    Zhang, Yongde
    Yang, Xueming
    GENES & GENOMICS, 2022, 44 (12) : 1487 - 1498