Comparative transcriptomic and physiological analyses reveal the key role of abscisic acid in hydrangea macrophylla responding to Corynespora cassiicola

被引：2

作者：

Chen, Huijie ^{[1
]}

Liu, Xintong ^{[1
]}

Mao, Jundan ^{[1
,2
]}

Qi, Xiangyu ^{[1
]}

Chen, Shuangshuang ^{[1
]}

Feng, Jing ^{[1
]}

Jin, Yuyan ^{[1
]}

Ahmad, Muhammad Zulfiqar ^{[1
]}

Sun, Ming ^{[3
]}

Deng, Yanming ^{[1
,3
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Leisure Agr, Jiangsu Prov Key Lab Genet & Improvement Hort Crop, Nanjing 210014, Peoples R China

[2] Anhui Univ Technol, Sch Architecture & Civil Engn, Maanshan 243032, Peoples R China

[3] Beijing Forestry Univ, State Key Lab Efficient Prod Forest Resources, Beijing 100083, Peoples R China

来源：

BMC PLANT BIOLOGY | 2024年 / 24卷 / 01期

关键词：

Hydrangea macrophylla; Leaf spot disease; Disease resistance evaluation; Transcriptome; ABA; DEFENSE RESPONSES; ABA; 8'-HYDROXYLASES; BIOSYNTHESIS; RESISTANCE; MECHANISMS; ETHYLENE; GENES;

D O I：

10.1186/s12870-024-05770-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

BackgroundBigleaf hydrangea (Hydrangea macrophylla) is a widely cultivated ornamental plant species. Leaf spot disease, caused by Corynespora cassiicola, poses a significant threat to the ornamental quality and economic value of hydrangeas. However, the disease resistance breeding of hydrangea is limited due to the lacking of resistant varieties and genes. ResultsThis study evaluated ten hydrangea varieties for their resistance to leaf spot disease. Among them, 'White Angel' and 'Ocean Heart' were screened out as representative varieties for resistance and susceptibility, respectively, on the basis of evaluation. Physiological and biochemical indices, phytohormones, and transcriptomic changes were measured in the leaves of both varieties at 0 and 24 h post inoculation with C. cassiicola. The results showed that C. cassiicola infection significantly increased abscisic acid (ABA) contents in both varieties; however, the increase was significantly higher in the susceptible variety 'Ocean Heart' compared to the resistant variety 'White Angel' (p < 0.05). Moreover, exogenous ABA (100 <mu>M) decreased the leaves' resistance to C. cassiicola of both varieties, underscoring its key role in reduced disease resistance. Transcriptome profiling revealed 17,087 differentially expressed genes (DEGs) responding to C. cassiicola between the two varieties. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated significant enrichment of DEGs in "Plant hormone signal transduction", particularly related to ABA signaling (HmPP2C and HmABFs). In addition, the expression of ABA biosynthesis genes (HmZEP3, HmABA2, and HmAAO3) was upregulated in both varieties. Meanwhile, the ABA catabolism gene (HmCYP707A4) exhibited significantly upregulated expression in the resistant variety 'White Angel' and downregulated expression in the susceptible variety 'Ocean Heart'. Intriguingly, the expression of HmCYP707A4 was 15-fold higher in 'White Angel' than in 'Ocean Heart'. ConclusionIn summary, these findings highlight the crucial role of ABA in the resistance of bigleaf hydrangea to leaf spot disease and provide valuable genetic resources for breeding programs to enhance the disease resistance in hydrangeas.

引用

页数：15