Evaluation of the Biological Properties of the Bacteriodaceae Family Using the Caco-2 Cell Line

被引：0
作者：
Podoprigora, I. V. [1 ]
Das, M. S. [1 ]
Zakharzhevskaya, N. B. [2 ]
Kafarskaya, L. I. [3 ]
Efimov, B. A. [2 ,3 ]
机构：
[1] Peoples Friendship Univ Russia, Inst Med, Moscow, Russia
[2] Fed Med Biol Agcy Russia, Lopukhin Fed Res & Clin Ctr Phys Chem Med, Moscow, Russia
[3] Pirogov Russian Natl Res Med Univ, Inst Prevent Med, Minist Hlth Russian Federat, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2024年 / 178卷 / 01期
基金：
俄罗斯科学基金会;
关键词：
Bacteroidaceae; adhesion; TEER; Caco-2; new biotherapeutic drugs;
D O I：
10.1007/s10517-024-06281-y
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Disorders in the composition of the microbiota are often associated with pathological processes in the body. The use of new biotherapeutic drugs containing bacteria from normal microflora to correct these disorders may contribute to more effective treatment. The Bacteroidaceae family dominating in microflora is a candidate for inclusion in bacterial preparations. The adhesion properties of five bacterial strains from the Bacteroidaceae family were analyzed using the Caco-2 cell line and their effect on the transepithelial electrical resistance parameters (TEER) was assessed to determine the extent of their impact on the cell monolayer integrity. This approach allowed the selection of strain B. intestinalis 181, which was characterized by a moderate adhesion activity combined with a relatively low effect on the TEER parameter.
引用
收藏
页码：54 / 57
页数：4
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