Next-generation sequencing in ophthalmology

被引：0

作者：

Wolf, Julian ^{[1
,2
,3
]}

Lange, Clemens ^{[1
,2
,4
]}

Reinhard, Thomas ^{[1
,2
]}

Schlunck, Guenther ^{[1
,2
]}

机构：

[1] Univ Klinikum Freiburg, Klin Augenheilkunde, Killianstr 5, D-79106 Freiburg, Germany

[2] Albert Ludwigs Univ Freiburg, Med Fak, Freiburg, Germany

[3] Stanford Univ, Byers Eye Inst, Sch Med, Palo Alto, CA USA

[4] Augenzentrum St Franziskus Hosp, Ophtha Lab, Munster, Germany

来源：

SPEKTRUM DER AUGENHEILKUNDE | 2024年 / 38卷 / 06期

关键词：

RNA sequencing; Transcriptome analysis; DNA sequencing; Genome; Macular degeneration;

D O I：

10.1007/s00717-024-00580-5

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

New methods for basic research are finding their way into ophthalmology and expand the options for research, diagnostics and treatment. This article centers on the study of gene activity in cells and tissues by next-generation sequencing (NGS). Following a brief introduction to the basic principles of NGS, this article focuses on transcriptome analysis by RNA sequencing using examples from ophthalmology. The RNA sequencing provides a comprehensive and unbiased overview of gene activity in cells and tissues and thus forms an important foundation for generating new testable scientific hypotheses. It thus contributes to the in-depth characterization of pathological changes and supports the development of new diagnostic and therapeutic approaches.

引用

页码：260 / 270

页数：11

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