Immobilization of Heavy Metals by Phosphorus-solubilizing Bacteria and Inhibition of Cd and Pb Uptake by Wheat

被引：0

作者：

Su N.-N. ^{[1
]}

Zhao Q.-Z. ^{[1
]}

Wang F. ^{[2
]}

An T.-C. ^{[1
]}

Niu J.-J. ^{[1
]}

Yan J.-X. ^{[1
]}

Yang J.-J. ^{[3
]}

Han H. ^{[1
,3
]}

机构：

[1] Henan Field Observation and Research Station of Headwork Wetland Ecosystem of the Central Route of South-to-North Water Diversion Project, Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water D

[2] Agricultural Technology Promotion Center in Qingyuan District, Baoding, Baoding City

[3] Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing

来源：

Huanjing Kexue/Environmental Science | 2024年 / 45卷 / 07期

关键词：

available phosphorus; heavy metal immobilization; microbial community; phosphorus-solubilizing bacteria; wheat;

D O I：

10.13227/j.hjkx.202308206

中图分类号：

学科分类号：

摘要：

Phosphorus-solubilizing microorganisms convert insoluble phosphorus in the soil into phosphorus that can be absorbed by plants. Soluble phosphate combines with heavy metals to form precipitation，reducing the content of available heavy metals，thereby reducing the absorption of heavy metals by crops，which plays an important role in the remediation of heavy metal-contaminated soil. The effects of the immobilization of Cd and Pb and the release of PO43− by the phosphorus-solubilizing bacterium Klebsiella sp. M2 were studied through solution culture experiments. In addition，the effects of strain M2 on wheat uptake of Cd and Pb and its microbiological mechanism were also explored through pot experiments. The results showed that strain M2 reduced the concentrations of Cd and Pb and increased the concentration of PO43− in the solution through cell wall adsorption and induced phosphate precipitation. Pot experiments showed that compared to those in the CK group and inactivated strain M2 group，inoculation with live strain M2 significantly increased（123%-293%）the contents of Ca2-P and Ca8-P in rhizosphere soil，decreased the content of DTPA-Cd（34. 48%）and DTPA-Pb（36. 72%）in wheat rhizosphere soil，and thus hindered the accumulation of Cd and Pb in wheat grains. Moreover，high-throughput sequencing results showed that strain M2 significantly increased the diversity of wheat rhizosphere bacterial communities；increased the relative abundance of Proteobacteria，Gemmatimonadetes，and Bacteroidota in wheat rhizosphere soil；and increased the proportion of heavy metal-immobilizing and phosphorus-promoting bacteria in wheat rhizosphere soil（mainly Sphingomonas，Nocardioides，Bacillus，Gemmatimonas，and Enterobacter）. These bacterial genera played an important role in immobilizing heavy metals and preventing wheat from absorbing heavy metals. These results provide bacterial resources and theoretical basis for the bioremediation of heavy metal-contaminated farmland. © 2024 Science Press. All rights reserved.

引用

页码：4321 / 4331

页数：10

共 46 条

[1] Yu Z，, Chen F，, Zhang J F，, Et al., Contamination and risk of heavy metals in soils and vegetables from zinc smelting area［J］, China Environmental Science, 39, 5, pp. 2086-2094, (2019)
[2] Leon-Canedo J A，, Alarcon-Silvas S G，, Fierro-Sanudo J F，, Et al., Mercury and other trace metals in lettuce（Lactuca sativa）grown with two low-salinity shrimp effluents：accumulation and human health risk assessment［J］, Science of the Total Environment, 650, pp. 2535-2544, (2019)
[3] Shi H Y, Wang P，, Zheng J T，, Et al., Spatial distribution of soil heavy metals and regional control strategies in China at province level ［J］, Environmental Science, 44, 8, pp. 4706-4716, (2023)
[4] Zeng X B, Xu J M, Huang Q Y，, Et al., Some deliberations on the issues of heavy metals in farmlands of China［J］, Acta Pedologica Sinica, 50, 1, pp. 186-194, (2013)
[5] Han H，, Wang X Y，, Cai H，, Et al., Isolation of heavy metal immobilizing and plant growth-promoting bacteria and its effects on reducing heavy metal accumulation in wheat［J］, Environmental Science, 40, 7, pp. 3339-3346, (2019)
[6] Li X F., Technical solutions for the safe utilization of heavy metal-contaminated farmland in China： a critical review ［J］, Land Degradation & Development, 30, 15, pp. 1773-1784, (2019)
[7] Guan W D，, Guo D, Wang P，, Et al., Investigations on the derivation of safe maize-producing threshold of soil Cd content and on classification of Cd contaminated maize-producing areas in northern China［J］, Environmental Science, 42, 12, pp. 5958-5966, (2021)
[8] Wang Y L，, Lambers H., Root-released organic anions in response to low phosphorus availability：recent progress，challenges and future perspectives［J］, Plant and Soil, 447, 1-2, pp. 135-156, (2020)
[9] Fang B，, Chen L，, Wang Y，, Et al., Accumulation，migration，and transformation of soil phosphorus in facility agriculture and its influencing factors［J］, Environmental Science, 44, 1, pp. 452-462, (2023)
[10] Ma S J，, Nan Z R，, Hu Y H，, Et al., Phosphorus supply level is more important than wheat variety in safe utilization of cadmium-contaminated calcareous soil［J］, Journal of Hazardous Materials, (2022)

← 1 2 3 4 5 →